Neal R A
Exp Parasitol. 1984 Jun;57(3):269-73. doi: 10.1016/0014-4894(84)90100-0.
Promastigotes of Leishmania major were isolated from an infected mouse in two media, blood agar and Schneider's medium + 30% fetal calf serum, and maintained continuously for over 1 year. Infectivity studies in two strains of mice, outbred CD1 strain and inbred BALB/c strain, showed that promastigotes grown in Schneider's medium maintained infectivity to BALB/c mice throughout the period of cultivation. Infectivity to CD1 strain mice was progressively lost. Promastigotes grown in blood agar medium, however, lost infectivity to both strains of mice at a faster rate than promastigotes grown in Schneider's medium.
从一只感染的小鼠中，在血琼脂和施奈德培养基+30%胎牛血清这两种培养基中分离出硕大利什曼原虫的前鞭毛体，并连续维持培养超过1年。在两种小鼠品系（远交系CD1品系和近交系BALB/c品系）中进行的感染性研究表明，在施奈德培养基中生长的前鞭毛体在整个培养期间对BALB/c小鼠保持感染性。对CD1品系小鼠的感染性逐渐丧失。然而，在血琼脂培养基中生长的前鞭毛体比在施奈德培养基中生长的前鞭毛体更快地丧失对这两种小鼠品系的感染性。
