Homayouni Mohamad Mohsen, Rostami Ali, Gholizadeh Hamed, Mehbod Amir Sayed Ali, Ebrahimi Maryam, Mehravar Saeed
Department of Parasitology and Mycology, School of Medicine, AJA University of Medical Sciences, Tehran, Iran.
Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Parasit Dis. 2017 Mar;41(1):298-301. doi: 10.1007/s12639-016-0776-3. Epub 2016 Jun 2.
pneumonia due to infection is an emerging health problem not only for HIV-infected patients but also for other immunocompromised patients in many countries. We compared Gomori methenamine silver (GMS), Toluidine Blue O (TBO) and Giemsa staining methods using standard procedures. The sensitivity and specificity of GMS were 100 %. The sensitivity and specificity of TBO were 96 and 100 %, respectively. The sensitivity and specificity of Giemsa stain were 84 and 90 %, respectively. Only GMS had positive and negative predictive values of 100 % while PPV and NPV for TBO were 100 and 90.9 %, and for Giemsa stain were 95.4 and 69.2 %, respectively. Therefore, our results suggest that if TBO or Geimsa stains are used as the primary staining methods in a clinical laboratory, then confirmation with a GMS staining method should be performed to increase the sensitivity and specificity of the final test result.
由感染引起的肺炎不仅在许多国家成为感染艾滋病毒患者面临的一个新出现的健康问题，也是其他免疫功能低下患者面临的问题。我们使用标准程序比较了高碘酸-希夫（GMS）、甲苯胺蓝O（TBO）和吉姆萨染色方法。GMS的敏感性和特异性均为100%。TBO的敏感性和特异性分别为96%和100%。吉姆萨染色的敏感性和特异性分别为84%和90%。只有GMS的阳性和阴性预测值为100%，而TBO的阳性预测值和阴性预测值分别为100%和90.9%，吉姆萨染色的阳性预测值和阴性预测值分别为95.4%和69.2%。因此，我们的结果表明，如果在临床实验室将TBO或吉姆萨染色用作主要染色方法，那么应采用GMS染色方法进行确认，以提高最终检测结果的敏感性和特异性。
