Lautenschlager I, Lyytikainen O, Jokipii L, Jokipii A, Maiche A, Ruutu T, Tukiainen P, Ruutu P
Laboratory Department, Helsinki University Central Hospital, Finland.
J Clin Microbiol. 1996 Mar;34(3):728-30. doi: 10.1128/jcm.34.3.728-730.1996.
Immunodetection of Pneumocystis carinii, based on immunofluorescence and use of a monoclonal antibody specific for an antigen located within the cyst wall and detectable after trypsin digestion only, was compared with a methenamine silver stain in 553 bronchoalveolar lavage specimens from immunosuppressed patients. P. carinii was found by immunofluorescence in 72 (86%) and by silver stain in 68 (81%) of the total of 84 positive samples detected by either or both of these methods.
在553份来自免疫抑制患者的支气管肺泡灌洗标本中,将基于免疫荧光并使用仅在胰蛋白酶消化后可检测到的、针对位于囊壁内抗原的单克隆抗体进行的卡氏肺孢子虫免疫检测,与亚甲胺银染色法进行了比较。在通过这两种方法中的任何一种或两种检测到的总共84份阳性样本中,通过免疫荧光法发现卡氏肺孢子虫的有72份(86%),通过银染色法发现的有68份(81%)。