支气管肺泡灌洗标本中卡氏肺孢子虫的免疫检测与亚甲胺银染色的比较
Immunodetection of Pneumocystis carinii in bronchoalveolar lavage specimens compared with methenamine silver stain.
作者信息
Lautenschlager I, Lyytikainen O, Jokipii L, Jokipii A, Maiche A, Ruutu T, Tukiainen P, Ruutu P
机构信息
Laboratory Department, Helsinki University Central Hospital, Finland.
出版信息
J Clin Microbiol. 1996 Mar;34(3):728-30. doi: 10.1128/jcm.34.3.728-730.1996.
Abstract
Immunodetection of Pneumocystis carinii, based on immunofluorescence and use of a monoclonal antibody specific for an antigen located within the cyst wall and detectable after trypsin digestion only, was compared with a methenamine silver stain in 553 bronchoalveolar lavage specimens from immunosuppressed patients. P. carinii was found by immunofluorescence in 72 (86%) and by silver stain in 68 (81%) of the total of 84 positive samples detected by either or both of these methods.
摘要
在553份来自免疫抑制患者的支气管肺泡灌洗标本中，将基于免疫荧光并使用仅在胰蛋白酶消化后可检测到的、针对位于囊壁内抗原的单克隆抗体进行的卡氏肺孢子虫免疫检测，与亚甲胺银染色法进行了比较。在通过这两种方法中的任何一种或两种检测到的总共84份阳性样本中，通过免疫荧光法发现卡氏肺孢子虫的有72份（86%），通过银染色法发现的有68份（81%）。
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