Jeong Seon Mi, Lee Hwa Jeong, Park Yoon Mee, Kim Jin Seok, Lee Sang Dae, Bang Iel Soo
Department of Microbiology and Immunology, Chosun University School of Dentistry, Gwangju 61452, Korea.
Division of Life Science, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Korea.
Korean J Food Sci Anim Resour. 2017;37(1):134-138. doi: 10.5851/kosfa.2017.37.1.134. Epub 2017 Feb 28.
infects a broad range of host animals, and zoonostic infection threatens both public health and the livestock and meat processing industries. Many antimicrobials have been developed to target envelope that performs essential bacterial functions; however, there are very few analytical methods that can be used to validate the efficacy of these antimicrobials. In this study, to develop a potential biosensor for envelope stress, we examined the transcription of the serovar gene, the ortholog of which in encodes Spy (spheroplast protein y). Spy is a chaperone protein expressed and localized in the periplasm of during spheroplast formation, or by exposure to protein denaturing conditions. expression in was examined by constructing a transcriptional fusion. transcription was strongly induced during spheroplast formation, and also when exposed to membrane-disrupting agents, including ethanol and the antimicrobial peptide polymyxin B. Moreover, induction required the activity of regulator proteins BaeR and CpxR, which are part of the major envelope stress response systems BaeS/BaeR and CpxA/CpxR, respectively. Results suggest that monitoring transcription may be useful to determine whether a molecule particularly cause envelope stress in .
可感染多种宿主动物，人畜共患感染对公共卫生以及畜牧业和肉类加工业都构成威胁。已经研发出许多针对细菌包膜发挥基本功能的抗菌药物；然而，可用于验证这些抗菌药物疗效的分析方法却很少。在本研究中，为开发一种潜在的用于检测包膜应激的生物传感器，我们检测了血清型基因的转录情况，该基因在中的直系同源基因编码Spy（球状体蛋白y）。Spy是一种伴侣蛋白，在球状体形成过程中或暴露于蛋白质变性条件下时表达并定位于的周质中。通过构建转录融合体来检测在中的表达情况。在球状体形成过程中以及暴露于包括乙醇和抗菌肽多粘菌素B在内的膜破坏剂时，转录会强烈诱导。此外，的诱导需要调节蛋白BaeR和CpxR的活性，它们分别是主要包膜应激反应系统BaeS/BaeR和CpxA/CpxR的一部分。结果表明，监测转录情况可能有助于确定一种分子是否特别会在中引起包膜应激。
