人源单克隆抗体对脊髓灰质炎病毒的型间交叉中和作用。

Intertypic cross-neutralization of polioviruses by human monoclonal antibodies.

作者信息

Uhlig H, Dernick R

机构信息

Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Universität Hamburg, Federal Republic of Germany.

出版信息

Virology. 1988 Mar;163(1):214-7. doi: 10.1016/0042-6822(88)90251-6.

DOI:10.1016/0042-6822(88)90251-6

PMID:2831657

Abstract

Human B cell lines producing monoclonal antibodies (mAbs) reactive with poliovirus type 1 were generated by transformation with Epstein-Barr virus (EBV) B 95-8 of tonsillar lymphocytes from several immune donors. EBV-transformed cells were cloned in semisolid agarose. Some neutralizing (Nt) human mAbs recognized and neutralized only poliovirus type 1, whereas other Nt mAbs neutralized either poliovirus type 1 and 2 or all three serotypes. mAbs reactive with poliovirus type 1 and 3 but not with type 2 were not detected. Immunoprecipitation of radiolabeled poliovirus type 1 with cross-reactive human Nt mAbs was inhibited competitively by preincubation of mAbs with cold poliovirus type 3 and/or 2.

摘要

通过用来自多个免疫供体的扁桃体淋巴细胞的爱泼斯坦 - 巴尔病毒（EBV）B95 - 8进行转化，产生了与1型脊髓灰质炎病毒反应的产生单克隆抗体（mAb）的人B细胞系。EBV转化的细胞在半固体琼脂糖中克隆。一些中和（Nt）人mAb仅识别并中和1型脊髓灰质炎病毒，而其他Nt mAb中和1型和2型脊髓灰质炎病毒或所有三种血清型。未检测到与1型和3型脊髓灰质炎病毒反应但与2型不反应的mAb。用交叉反应性人Nt mAb对放射性标记的1型脊髓灰质炎病毒进行免疫沉淀，可通过将mAb与冷的3型和/或2型脊髓灰质炎病毒预孵育而竞争性抑制。