Two new pterocarpans and a new pyrone derivative with cytotoxic activities from (N.E.Br.) Brummitt (Leguminosae): revised NMR assignment of mundulea lactone.

BACKGROUND

is a genus related to which comprises only three species. Compared to , which has been phytochemically and pharmacologically studied, species have only few or no reports on their chemical constituents. Moreover, no studies on the cytotoxic activities of its secondary metabolites have been previously documented.

RESULTS

From the non polar fractions of the roots bark of (syn ), two new pterocarpans: seputhecarpan C and seputhecarpan D and a new pyrone derivative, ptycholopyrone A were isolated. Alongside, five known compounds identified as 3-α,α-dimethylallyl-4-methoxy-6-styryl-α-pyrone or mundulea lactone , glyasperin F , seputhecarpan A , seputheisoflavone and 5--methyl-myo-inositol or sequoyitol were also obtained. Their structures were established by the mean means of spectroscopic data in conjunction to those reported in literature. The NMR assignment of the major compound mundulea lactone is revised in this paper. In addition, the cytotoxicity of the isolated metabolites was evaluated on two lung cancer cell lines A549 and SPC212. was not active while compounds displayed antiproliferative effects against the two carcinoma cell lines with IC values below 75 µM. IC values below 10 µM were obtained for and on SPC212 cells.

CONCLUSION

Based on the obtained results, turns to be a rich source of phenolic metabolites among them some bearing prenyl moieties. This study reports for the first time the isolation of pyrone derivatives and from genus. The cytotoxicity observed for the isolate is also reported for the first time and shows that and could be chemically explored in order to develop a hit candidate against lung cancer. Graphical abstractTwo new pterocarpans and a new pyrone derivative with cytotoxic activities from ptycholobium contortum (N.E.Br.) Brummitt (Leguminosae): revised NMR assignment of mundulea lactone.