使用单一微型角膜刀一次切割制备用于Descemet膜剥除内皮角膜移植术的超薄植片。

Preparation of ultrathin grafts for Descemet-stripping endothelial keratoplasty with a single microkeratome pass.

作者信息

Romano Vito, Steger Bernhard, Myneni Jayavani, Batterbury Mark, Willoughby Colin E, Kaye Stephen B

机构信息

From the Department of Corneal and External Eye Diseases (Romano, Myneni, Batterbury, Willoughby, Kaye), St. Paul's Eye Unit, Royal Liverpool University Hospital, and the Department of Eye and Vision Science (Willoughby, Kaye), University of Liverpool, Liverpool, United Kingdom; the Department of Ophthalmology (Steger), Medical University of Innsbruck, Innsbruck, Austria.

出版信息

J Cataract Refract Surg. 2017 Jan;43(1):12-15. doi: 10.1016/j.jcrs.2016.12.009.

DOI:10.1016/j.jcrs.2016.12.009

PMID:28317665

Abstract

We present a technique to achieve ultrathin Descemet-stripping automated endothelial keratoplasty (DSAEK). Using a simple method of controlling artificial anterior chamber pressure and drying the corneal surface, it was possible to thin the donor cornea at a rate of 11 μm a minute. When the donor cornea was between 500 μm and 510 μm, a single pass was made using a 350 μm microkeratome head followed by a peripheral dissection. The resulting mean graft thickness was 83.2 μm ± 14.9 (SD) (range 50 to 98 μm) with a mean peripheral graft edge thickness of 106.8 ± 10.9 μm (range 90 to 120 μm). There were no surgical complications, and all grafts remained attached. This is a reliable method for preparing ultrathin donor corneal lenticules for DSAEK in the operating room or eye bank without using multiple microkeratome heads or risking double passes.

摘要

我们介绍一种实现超薄后弹力层剥除自动内皮角膜移植术（DSAEK）的技术。通过一种控制人工前房压力和干燥角膜表面的简单方法，能够以每分钟11μm的速度使供体角膜变薄。当供体角膜厚度在500μm至510μm之间时，使用350μm的微型角膜刀头进行单次切割，随后进行周边剥离。最终移植片的平均厚度为83.2μm±14.9（标准差）（范围为50至98μm），移植片周边边缘的平均厚度为106.8±10.9μm（范围为90至120μm）。未出现手术并发症，所有移植片均保持附着。这是一种在手术室或眼库中为DSAEK制备超薄供体角膜透镜的可靠方法，无需使用多个微型角膜刀头或冒着进行两次切割的风险。