[活检对Tca8113移植瘤生物学行为影响的探索性研究]
[Exploratory study on influence of biopsy to biological behavior of Tca8113 transplanted tumor].
作者信息
Jiyuan Liu, Jian Pan, Chengge Hua, Yunlong Wu, Zhengwen Luo, Xiufa Tang
机构信息
State Key Laboratory of Oral Diseases, Dept. of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
State Key Laboratory of Oral Diseases, Dept. of Head and Neck Oncology, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
出版信息
Hua Xi Kou Qiang Yi Xue Za Zhi. 2016 Dec 1;34(6):626-631. doi: 10.7518/hxkq.2016.06.015.
OBJECTIVE
We established an animal model of nude mice with Tca8113 tumor and cut some tissue for biopsy. We also determined the biological behavior and mechanisms of the tumor.
METHODS
The mice were divided into two groups randomly. Mice in both groups were injected with Tca8113 cells into their tongues. The survival condition, growth of primary focus, and metastasis were observed. Hematoxylin and eosin staining and immunohistochemistry were performed on nuclear factor κB (NF-κB), matrix metallopeptidase 9 (MMP-9), vascular endothelial growth factor (VEGF), stromal cell-derived factor 1 (SDF-1), and Ki67 to determine their distributions within the tumor. Cytokeratin staining was also performed to detect micrometastasis in the submandibular lymph nodes.
RESULTS
The emerging rate of tumor was 97.92%. The weight and survival time of the experimental group were lower than that of the control group, whereas the metastasis ratio was higher. The expression of NF-κB, MMP-9, SDF-1, and MMP-9 in tumors was higher in the experimental group than that in the control group. The expression of NF-κB, MMP-9, VEGF, and SDF-1 was relevant. The microvessel density of the experimental group was higher than that in the control group.
CONCLUSIONS
Biopsy can affect the biological behavior of tongue tumor and can promote growth of primary focus and metastasis.
目的
建立Tca8113肿瘤裸鼠动物模型并切取部分组织进行活检，同时确定肿瘤的生物学行为及机制。
方法
将小鼠随机分为两组，两组小鼠均经舌部注射Tca8113细胞，观察其生存状况、原发灶生长及转移情况。对核因子κB（NF-κB）、基质金属蛋白酶9（MMP-9）、血管内皮生长因子（VEGF）、基质细胞衍生因子1（SDF-1）和Ki67进行苏木精-伊红染色及免疫组化，以确定它们在肿瘤内的分布。还进行细胞角蛋白染色以检测下颌下淋巴结中的微转移。
结果
肿瘤出现率为97.92%。实验组小鼠的体重和生存时间低于对照组，而转移率更高。实验组肿瘤中NF-κB、MMP-9、SDF-1和MMP-9的表达高于对照组。NF-κB、MMP-9、VEGF和SDF-1的表达相关。实验组的微血管密度高于对照组。
结论
活检可影响舌肿瘤的生物学行为，促进原发灶生长及转移。
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