D'Amico Verónica L, Palacios María G, Baker Allan J, González Patricia M, Madrid Enrique, Bertellotti Marcelo
1 Centro para el Estudio de Sistemas Marinos, Centro Nacional Patagónico, Bvrd. Brown 2915, Puerto Madryn CP 9120, Chubut, Argentina.
2 Royal Ontario Museum, 100 Queens Park, Toronto, Canada.
J Wildl Dis. 2017 Jul;53(3):437-446. doi: 10.7589/2016-02-039. Epub 2017 Mar 20.
Physiologic traits are promising indicators of population health in the face of rapidly changing environments. We obtained values of diverse physiologic parameters for Two-banded Plovers (Charadrius falklandicus) in coastal sites in Patagonia, Argentina, with the objectives of determining the timeline in which these parameters become affected by the stress of capture and handling and of obtaining reference values for future monitoring of these populations. We analyzed packed cell volume, white blood cell profile, heterophil/lymphocyte ratio, bacterial agglutination titer, and total protein, glucose, triglyceride, and cholesterol levels in apparently healthy birds. Glucose, total white blood cell count, lymphocytes, and eosinophil levels showed changes with handling times >60 min after capture. The remaining parameters did not manifest significant alterations in response to capture and handling of up to 232 min (average=105.2, SD=56.7). Therefore, although researchers should attempt to obtain blood samples as soon as possible after capture, inclusion of physiologic parameters in monitoring studies of species not easily sampled in a few minutes, such as Two-banded Plovers and other shorebird species during migration, should not be discouraged. Here we provide a physiologic report for the species that can be considered as reference values during the nonbreeding season at Patagonian coastal sites.
面对迅速变化的环境，生理特征是种群健康状况的重要指标。我们获取了阿根廷巴塔哥尼亚沿海地区双带鸻（Charadrius falklandicus）各种生理参数的值，目的是确定这些参数在何时受到捕获和处理应激的影响，并获取未来监测这些种群的参考值。我们分析了看似健康的鸟类的红细胞压积、白细胞谱、嗜异性粒细胞/淋巴细胞比率、细菌凝集效价以及总蛋白、葡萄糖、甘油三酯和胆固醇水平。葡萄糖、白细胞总数、淋巴细胞和嗜酸性粒细胞水平在捕获后处理时间超过60分钟时出现变化。其余参数在长达232分钟（平均=105.2，标准差=56.7）的捕获和处理过程中未表现出显著变化。因此，尽管研究人员应在捕获后尽快采集血样，但在迁移期间不易在几分钟内采样的物种（如双带鸻和其他滨鸟物种）的监测研究中纳入生理参数，不应受到阻碍。在此，我们提供了该物种在巴塔哥尼亚沿海地区非繁殖季节可作为参考值的生理报告。
