Sakharov I Y, Dukhanina E A, Molokoedov A S, Danilov S M, Ovchinnikov M V, Bespalova Z D, Titov M I
Institute of Experimental Cardiology, CRC AMS USSR, Moscow.
Biochem Biophys Res Commun. 1988 Feb 29;151(1):109-13. doi: 10.1016/0006-291x(88)90565-7.
Hydrolysis of Bz-Gly-Ser-Phe-Arg, C-terminal fragment of atriopeptin 2, by human cardiac angiotensin-converting enzyme has been studied. The KM for the reaction was 10(-4) M. The effect of concentration of NaCl on activity of cardiac angiotensin-converting enzyme has been determined, which allowed to regard Bz-Gly-Ser-Phe-Arg as bradykinin-like substrates. It was demonstrated that cardiac, but not pulmonary isozyme of angiotensin-converting enzyme specifically hydrolyses atriopeptin 2.
