Toth C R, Walmsley J A
Department of Chemistry, University of Toledo, Ohio 43606.
Biochem Biophys Res Commun. 1988 Feb 29;151(1):86-93. doi: 10.1016/0006-291x(88)90562-1.
The tetramethylammonium salt of guanylyl-(3'-5')-guanosine has been prepared by a cation-exchange technique and it has been found that the tetramethylammonium ion drastically reduces the self-association of GpG in solution. This has allowed the characterization of GpG by FTIR and 1-D and 2-D NMR spectroscopy. A complete, well-resolved 1H NMR spectrum in D2O has been obtained and all resonances have been assigned. A weak, essentially non-cooperative intermolecular association is observed in solution (15-20 mM) below 40 degrees C. The association occurs via base stacking and base-base hydrogen bonding.
鸟苷酰基-（3'-5'）-鸟苷的四甲基铵盐已通过阳离子交换技术制备，并且发现四甲基铵离子能显著降低溶液中GpG的自缔合。这使得通过傅里叶变换红外光谱（FTIR）以及一维和二维核磁共振光谱对GpG进行表征成为可能。已在重水中获得了完整、分辨率良好的1H核磁共振谱，并对所有共振峰进行了归属。在40℃以下的溶液（15 - 20 mM）中观察到一种微弱的、基本非协同的分子间缔合。这种缔合通过碱基堆积和碱基间氢键发生。
