Lee M S, King C M
Chem Biol Interact. 1981 Mar 1;34(2):239-48. doi: 10.1016/0009-2797(81)90135-6.
N-Acetoxy-4-trifluoroacetylaminobiphenyl (N-acetoxy-TFAABP) reacted readily with Guo and GMP at neutrality in a one-step fashion to yield N-(guanosin-8-yl)4-aminobiphenyl (Guo-ABP) (I) and N(guanosin-8-yl)-4-aminobiphenyl-5'-monophosphate (GMP-ABP) (II), respectively. GMP-ABP could also be formed in much lower yield from the reaction of N-acetoxy-4-formylaminobiphenyl (N-acetoxy-FABP) with GMP (pH 7.0) under more rigorous conditions. Enzymatic hydrolysis of GMP-ABP with alkaline phosphatase in Tris buffer (pH 8.0) at 37 degrees C yielded Guo-ABP. Guo-ABP showed a brilliant blue fluorescence on exposure to 366 nm UV light and its UV absorption spectrum was identical to that of Guo-ABP prepared by Kriek via a different route. Elemental analysis and nuclear magnetic resonance (NMR) data further confirmed the identity of this compound.
N-乙酰氧基-4-三氟乙酰氨基联苯（N-乙酰氧基-TFAABP）在中性条件下能与鸟苷（Guo）和鸟苷-5'-单磷酸（GMP）一步反应，分别生成N-（鸟苷-8-基）-4-氨基联苯（Guo-ABP）（I）和N-（鸟苷-8-基）-4-氨基联苯-5'-单磷酸（GMP-ABP）（II）。在更严格的条件下，N-乙酰氧基-4-甲酰氨基联苯（N-乙酰氧基-FABP）与GMP（pH 7.0）反应也能生成产率低得多的GMP-ABP。在37℃下，于Tris缓冲液（pH 8.0）中用碱性磷酸酶对GMP-ABP进行酶促水解，生成Guo-ABP。Guo-ABP在366nm紫外光照射下呈现亮蓝色荧光，其紫外吸收光谱与Kriek通过不同途径制备的Guo-ABP相同。元素分析和核磁共振（NMR）数据进一步证实了该化合物的结构。
