Vidyarthi Aurobind, Khan Nargis, Agnihotri Tapan, Siddiqui Kaneez F, Nair Girish R, Arora Ashish, Janmeja Ashok K, Agrewala Javed N
CSIR-Institute of Microbial Technology, Chandigarh, India.
CSIR-Central Drug Research Institute, Lucknow, India.
PLoS One. 2017 Mar 20;12(3):e0173769. doi: 10.1371/journal.pone.0173769. eCollection 2017.
Tuberculosis continues to be one of the most devastating global health problem. Its diagnosis will benefit in timely initiation of the treatment, cure and therefore reduction in the transmission of the disease. Tests are available, but none can be comprehensively relied on for its diagnosis; especially in TB-endemic zones. PhoP is a key player in Mycobacterium tuberculosis virulence but nothing has been known about its role in the diagnosis of TB. We monitored the presence of anti-PhoP antibodies in the healthy, patients and their contacts. In addition, we also measured antibodies against early secretory antigens ESAT-6 and CFP-10, and latency associated antigen Acr-1 to include proteins that are associated with the different stages of disease progression. Healthy subjects showed high antibody titer against PhoP than patients and their contacts. In addition, a distinct pattern in the ratio of Acr-1/PhoP was observed among all cohorts. This study for the first time demonstrates a novel role of anti-PhoP antibodies, as a possible marker for the diagnosis of TB and therefore will contribute in the appropriate action and management of the disease.
结核病仍然是最具破坏性的全球健康问题之一。其诊断有助于及时开始治疗、治愈疾病，从而减少疾病传播。现有多种检测方法，但没有一种能完全依赖于结核病的诊断，尤其是在结核病流行地区。PhoP是结核分枝杆菌毒力的关键因素，但对其在结核病诊断中的作用尚不清楚。我们监测了健康人、患者及其接触者体内抗PhoP抗体的存在情况。此外，我们还检测了针对早期分泌抗原ESAT-6和CFP-10以及潜伏相关抗原Acr-1的抗体，这些蛋白质与疾病进展的不同阶段相关。健康受试者体内抗PhoP抗体滴度高于患者及其接触者。此外，在所有队列中均观察到Acr-1/PhoP比值的独特模式。本研究首次证明了抗PhoP抗体的新作用，作为结核病诊断的可能标志物，因此将有助于对该疾病采取适当的措施和管理。
