Laboratorio de Inmunología de Enfermedades Infecciosas, Instituto de Biomedicina "Dr. Jacinto Convit", Universidad Central de Venezuela, Caracas 1050-58, Venezuela.
Departamento de Hematología, Hospital Clínic, IDIBAPS, Barcelona 08036, Spain.
Dis Markers. 2021 Jul 6;2021:6673250. doi: 10.1155/2021/6673250. eCollection 2021.
Tuberculosis (TB) is being underdetected in children as most are smear-negative. This work was aimed at evaluating ESAT-6 and Ag85A synthetic peptides' serodiagnostic potential for diagnosing children having a clinical suspicion of TB.
The study involved 438 children: 77 Creole nonindigenous (13 suspected of having TB and 64 healthy ones) and 361 Warao indigenous children (39 suspected of TB and 322 healthy children). The approach's diagnostic information was compared using operational characteristics and receiver-operating characteristic (ROC) curves.
Ag85A P-29879 had 94.6% sensitivity (AUC = 0.741: 0.651 to 0.819 95% CI) in indigenous children. ESAT-6 P-12036 and P-12037 had 100% and 92.3% of sensitivity (AUC = 0.929: 0.929: 0.846 to 0.975 95% CI and 0.791: 63.9 to 98.7 95% CI, respectively) in Creole children. ESAT-6 peptides also allowed a differentiation between children with TB and healthy ones.
Further validation of this approach could lead to developing a complementary tool for rapid TB diagnosis in children.
由于大多数儿童的痰涂片检查为阴性，导致结核病（TB）在儿童中漏诊。本研究旨在评估 ESAT-6 和 Ag85A 合成肽的血清学诊断潜力，以诊断临床疑似结核病的儿童。
该研究纳入了 438 名儿童：77 名克里奥尔非土著儿童（13 名疑似结核病和 64 名健康儿童）和 361 名瓦劳土著儿童（39 名疑似结核病和 322 名健康儿童）。使用操作特性和接收者操作特征（ROC）曲线比较了该方法的诊断信息。
Ag85A P-29879 在土著儿童中的敏感性为 94.6%（AUC = 0.741：0.651 至 0.819 95%CI）。ESAT-6 P-12036 和 P-12037 在克里奥尔儿童中的敏感性分别为 100%和 92.3%（AUC = 0.929：0.929：0.846 至 0.975 95%CI 和 0.791：63.9 至 98.7 95%CI）。ESAT-6 肽还可区分结核病患儿和健康儿童。
进一步验证该方法可能会开发出一种用于儿童快速诊断结核病的补充工具。
