Lee Hyesook, Shim Whuisu, Kim Chae Eun, Choi So Yeon, Lee Haeshin, Yang Jaewook
T2B Infrastructure Center for Ocular Diseases, Inje University Busan Paik Hospital, Busanjin-gu, Busan, Republic of Korea.
Department of Chemistry (BK21 Plus Program), Korea Advanced Institute of Science and Technology (KAIST), Yuseong, Daejeon, Republic of Korea.
Invest Ophthalmol Vis Sci. 2017 Mar 1;58(3):1682-1691. doi: 10.1167/iovs.16-20843.
We investigated the possibility of the nanocomplex of poly(ethylene glycol) (PEG) and catechin as a new biomedical material to treat dry eye disease.
NOD.B10.H2b mice were exposed to an air draft and injected with scopolamine for 10 days. Ten days later, the mice were treated with normal saline (n = 11), 1% catechin (n = 11), 1% PEG (n = 11), and 1% catechin/PEG nanocomplex solution mixture containing catechin and PEG at weight ratios of 1:1 (CP1, n = 11), 1:5 (CP5, n = 11), and 1:10 (CP10, n = 11). All treatments were administered five times a day for 10 days. We estimated the effect of PEG/catechin nanocomplexes on inflammation, tear production, epithelium stabilization, and goblet cell density.
Desiccation stress significantly decreased tear production and increased the corneal irregularity score. Furthermore, desiccation stress markedly increased the detached epithelium and decreased the numbers of conjunctival goblet cells. In addition, the expression of proinflammatory-related factors was markedly induced by desiccation stress in the lacrimal glands. However, the PEG/catechin nanocomplex effectively induced an increase in tear production, stabilization of the corneal epithelium, and an increase in conjunctival goblet cells and anti-inflammatory improvements in a PEG dose-dependent manner.
In this study, we found that PEG may increase bioavailability of catechin. Therefore, the PEG/catechin nanocomplex can be used as a new biomedical material to treat dry eye disease through stabilization of the tear film and inhibition of inflammation.
我们研究了聚乙二醇（PEG）与儿茶素的纳米复合物作为一种治疗干眼症的新型生物医学材料的可能性。
将NOD.B10.H2b小鼠置于气流中，并注射东莨菪碱10天。10天后，用生理盐水（n = 11）、1%儿茶素（n = 11）、1% PEG（n = 11）以及儿茶素与PEG重量比为1:1（CP1，n = 11）、1:5（CP5，n = 11）和1:10（CP10，n = 11）的1%儿茶素/PEG纳米复合溶液混合物对小鼠进行治疗。所有治疗均每天给药5次，持续10天。我们评估了PEG/儿茶素纳米复合物对炎症、泪液分泌、上皮稳定性和杯状细胞密度的影响。
干燥应激显著降低了泪液分泌并增加了角膜不规则度评分。此外，干燥应激显著增加了上皮脱落并减少了结膜杯状细胞的数量。另外，干燥应激在泪腺中显著诱导了促炎相关因子的表达。然而，PEG/儿茶素纳米复合物以PEG剂量依赖的方式有效诱导了泪液分泌增加、角膜上皮稳定、结膜杯状细胞增加以及抗炎改善。
在本研究中，我们发现PEG可能会提高儿茶素的生物利用度。因此，PEG/儿茶素纳米复合物可作为一种新型生物医学材料，通过稳定泪膜和抑制炎症来治疗干眼症。
