Shanghai Key Laboratory of Bio-energy Crops, School of Life Sciences, Shanghai University, 99 Shangda Road, Shanghai 200444, China.
Shanghai Key Laboratory of Bio-energy Crops, School of Life Sciences, Shanghai University, 99 Shangda Road, Shanghai 200444, China; Energy Research Institute of Shandong Academy of Science, Jinan 250014, China.
Bioresour Technol. 2017 Jun;234:208-216. doi: 10.1016/j.biortech.2017.02.127. Epub 2017 Mar 4.
Effects of different cellulases on the release of phenolic acids from rice straw during saccharification were investigated in this study. All cellulases tested increased the contents of phenolic acids during saccharification. However, few free phenolic acids were detected, as they were present in conjugated form after saccharification when the cellulases from Trichoderma reesei, Trichoderma viride and Aspergillus niger were used. On the other hand, phenolic acids were present in free form when the Acremonium cellulolyticus cellulase was used. Assays of enzyme activity showed that, besides high cellulase activity, the A. cellulolyticus cellulase exhibited high feruloyl esterase (FAE) activity. A synergistic interaction between FAE and cellulase led to the increase in free phenolic acids, and thus an increase in antioxidative and antiradical activities of the phenolic acids. Moreover, a cost estimation demonstrated the feasibility of phenolic acids as value-added products to reduce the total production cost of ethanol.
本研究探讨了不同纤维素酶对水稻秸秆糖化过程中酚酸释放的影响。所有测试的纤维素酶都在糖化过程中增加了酚酸的含量。然而，检测到的游离酚酸很少，因为当使用里氏木霉、绿色木霉和黑曲霉的纤维素酶时，它们在糖化后以共轭形式存在。另一方面，当使用绿色木霉纤维素酶时，酚酸以游离形式存在。酶活性测定表明，除了具有高纤维素酶活性外，Acremonium cellulolyticus 纤维素酶还表现出高的阿魏酸酯酶（FAE）活性。FAE 和纤维素酶之间的协同作用导致游离酚酸增加，从而增加了酚酸的抗氧化和清除自由基活性。此外，成本估算表明，酚酸作为附加值产品具有可行性，可以降低乙醇的总生产成本。
