Department of Applied Sciences, College of Environmental Technology, Muroran Institute of Technology, 27-1 Mizumoto, Muroran, Hokkaido 050-8585, Japan.
Research Center for Environmentally Friendly Materials Engineering, Muroran Institute of Technology, 27-1 Mizumoto-cho, Muroran, Hokkaido 050-8585, Japan.
Bioresour Technol. 2017 Jun;234:99-105. doi: 10.1016/j.biortech.2017.03.008. Epub 2017 Mar 4.
Synthetic wastewater (SW) at various carbon concentrations (5-60g/l) were evaluated for polyhydroxyalkanoates (PHA) production using the bacteria Pseudomonas pseudoflava. Bacteria showed highest PHA production with 20g/l (57±5%), and highest carbon removal at 5g/l (74±6%) concentrations respectively. Structure, molecular weight, and thermal properties of the produced PHA were evaluated using various analytical techniques. Bacteria produced homo-polymer [poly-3-hydroxybutyrate (P3HB)] when only acetate was used as carbon source; and it produced co-polymer [poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) P(3HB-co-3HV)] by addition of co-substrate propionate. PHA synthase, the enzyme which produce PHA was extracted from two bacterial strains i.e., P. pseudoflava and P. palleronii and its molecular weight was analysed using SDS-PAGE. Protein concentration, and PHA synthase enzyme activity of P. pseudoflava and P. palleronii was carried out using spectrophotometer. Results denoted that P. pseudoflava can be used for degradation of organic carbon persistent in wastewaters and their subsequent conversion into PHA.
用假单胞菌假浮游动杆菌（Pseudomonas pseudoflava）评估了不同碳浓度（5-60g/l）的合成废水（SW）用于生产聚羟基烷酸酯（PHA）。细菌在 20g/l（57±5%）和 5g/l（74±6%）浓度下分别表现出最高的 PHA 产量和最高的碳去除率。使用各种分析技术评估了所产生的 PHA 的结构、分子量和热性能。当仅使用乙酸盐作为碳源时，细菌产生均聚物[聚-3-羟基丁酸酯（P3HB）]；通过添加共底物丙酸，它产生共聚物[聚-（3-羟基丁酸酯-共-3-羟基戊酸酯）P（3HB-co-3HV）]。PHA 合酶是产生 PHA 的酶，从两种细菌即假单胞菌假浮游动杆菌（P. pseudoflava）和假单胞菌帕罗尼（P. palleronii）中提取，并使用 SDS-PAGE 分析其分子量。使用分光光度计进行了 P. pseudoflava 和 P. palleronii 的蛋白浓度和 PHA 合酶酶活性的测定。结果表明，假单胞菌假浮游动杆菌可用于降解废水中持久的有机碳，并将其随后转化为 PHA。
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