Carmena M J, Recio M N, Prieto J C
Departamento de Bioquímica, Universidad de Alcalá de Henares, Madrid, Spain.
Biochim Biophys Acta. 1988 Apr 2;969(1):86-90. doi: 10.1016/0167-4889(88)90091-2.
The interaction of vasoactive intestinal peptide (VIP) with prostatic epithelial cells was studied after castration and testosterone replacement in pubertal and mature rats. The number of VIP receptors (but not the affinity) decreased 2 days after castration and returned to normal when subsequently treated with testosterone for 4 days. However, the stimulatory effect of VIP upon cyclic AMP accumulation was unaffected by the androgen withdrawal elicited by the surgical procedure. The results suggest the importance of androgens in the biosynthesis of VIP receptors and also in their coupling to adenylate cyclase by affecting the membrane fluidity.
在青春期和成年大鼠去势及睾酮替代后，研究了血管活性肠肽（VIP）与前列腺上皮细胞的相互作用。去势2天后VIP受体数量（而非亲和力）减少，随后用睾酮治疗4天则恢复正常。然而，VIP对环磷酸腺苷积累的刺激作用不受手术引起的雄激素撤除影响。结果表明雄激素在VIP受体生物合成中以及通过影响膜流动性在其与腺苷酸环化酶偶联中的重要性。
