Viktorov A V, Dank E Kh, Kuznetsov V A, Ter-Simonian V G, Iurkiv V A
Biull Eksp Biol Med. 1988 Feb;105(2):158-60.
The preincubation of isolated washed human platelets with lipopolysaccharide toxin (LPS) from Salmonella typhimurium (10 min, 37 degrees) speeds up the thrombin-induced aggregation. Besides, LPS-pretreatment was shown to alter the turnover of polyphosphoinositides stimulated by thrombin and significantly elevated concentrations of diacylglycerol and thromboxane B2 in comparison with control platelets. However, LPS does not influence on the lipid fluidizing effect of thrombin action as testifies by ESR spectroscopy with doxyl-stearic acids as spin-probes. An LPS pretreatment of platelets that induces no aggregation by itself is proposed might transform cells into a state of hidden activation" associated with the accumulation of such products as diacylglycerol and precursors of thromboxane B2. Addition of thrombin to such "preactivated" platelets could cause a more effective aggregation.
将分离并洗涤过的人血小板与鼠伤寒沙门氏菌的脂多糖毒素（LPS）进行预孵育（10分钟，37摄氏度）可加速凝血酶诱导的聚集。此外，与对照血小板相比，LPS预处理显示可改变凝血酶刺激的多磷酸肌醇的周转，并显著提高二酰基甘油和血栓素B2的浓度。然而，如以多羟基硬脂酸作为自旋探针的电子顺磁共振光谱所证明的，LPS并不影响凝血酶作用的脂质流化效应。有人提出，对血小板进行LPS预处理本身虽不诱导聚集，但可能会使细胞转变为“隐藏激活”状态，这与二酰基甘油和血栓素B2前体等产物的积累有关。向这种“预激活”的血小板中添加凝血酶可能会导致更有效的聚集。
