Department of Operative Dentistry, Endodontics and Dental Materials, School of Dentistry of Bauru, University of São Paulo, Bauru, São Paulo, Brazil; Department of Restorative Dentistry, Federal University of Paraná, Curitiba, Paraná, Brazil.
Department of Operative Dentistry, Endodontics and Dental Materials, School of Dentistry of Bauru, University of São Paulo, Bauru, São Paulo, Brazil.
J Endod. 2017 May;43(5):779-786. doi: 10.1016/j.joen.2016.12.023. Epub 2017 Mar 18.
This study evaluated the physicochemical, antimicrobial, and biological properties of white mineral trioxide aggregate (MTA) mixed with aqueous and propylene glycol extracts of Arctium lappa L. and Casearia sylvestris Sw.
The setting-time test was performed according to American Society for Testing and Materials 266/2008 and flowability by American National Standards Institute/American Dental Association 57/2012 standard specifications. The following parameters were assessed: volumetric change by micro-computed tomography; pH level and calcium ion release measured after 3 hours and 1, 3, 7, and 15 days; antimicrobial effect by the dentin decontamination method with intratubular Enterococcus faecalis viability, verified by using confocal laser scanning microscopy; and biocompatibility by histologic-morphometric analyses of inflammatory infiltrate at subcutaneous implant sites after 15, 30, and 60 days. In addition, rat alveolar tissues with implants were processed to measure tumor necrosis factor-alpha and interleukin-10 cytokines by enzyme-linked immunosorbent assay.
The plant extracts associated with MTA significantly increased the final setting time; however, they did not influence volumetric change (P > .05) and maintained medium alkalinity and calcium ion release. Propylene glycol extracts showed higher flowability. Casearia sylvestris increased the cementing effect against E. faecalis after 24-hour and 168-hour periods. Histologic evaluation of inflammatory infiltrate showed no significant differences between plant extracts groups and the distilled water group for all periods. Tumor necrosis factor-alpha and interleukin-10 expression was similar among groups (P > .05).
Casearia sylvestris extracts increased the antimicrobial effect of MTA and did not influence biocompatibility but changed some physicochemical properties.
本研究评估了白色矿物三氧化物聚合体（MTA）与牛蒡根和山黄皮的水提物和丙二醇提取物混合后的理化性质、抗菌性能和生物学性能。
根据美国材料试验协会 266/2008 标准和美国国家标准协会/美国牙科协会 57/2012 标准规范进行凝固时间测试和流动性测试。评估以下参数：通过微计算机断层扫描测量体积变化；在 3 小时和 1、3、7 和 15 天测量 pH 值和钙离子释放量；通过管内粪肠球菌活力的牙本质去污法评估抗菌效果，并用共聚焦激光扫描显微镜进行验证；通过皮下植入部位炎症浸润的组织形态计量学分析评估生物相容性，在 15、30 和 60 天进行测量。此外，对植入物的大鼠肺泡组织进行处理，通过酶联免疫吸附试验测量肿瘤坏死因子-α和白细胞介素-10 细胞因子。
MTA 与植物提取物联合使用显著增加最终凝固时间；然而，它们不影响体积变化（P>.05），并保持中等碱性和钙离子释放。丙二醇提取物显示出更高的流动性。山黄皮在 24 小时和 168 小时后增加了对粪肠球菌的粘结效果。在所有时期，炎性浸润的组织学评价显示植物提取物组与蒸馏水组之间无显著差异。肿瘤坏死因子-α和白细胞介素-10 的表达在各组之间相似（P>.05）。
山黄皮提取物增加了 MTA 的抗菌效果，不影响生物相容性，但改变了一些理化性质。
