Midena Raquel Zanin, Garcia Roberto Brandão, Cavenago Bruno Cavalini, Marciano Marina Angélica, Minotti Paloma Gagliardi, Ordinola-Zapata Ronald, Weckwerth Paulo Henrique, Andrade Flaviana Bombarda de, Duarte Marco Antonio Hungaro
Department of Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.
Health Sciences Department, University of Sagrado Coração, Bauru, SP, Brazil.
J Appl Oral Sci. 2015 Oct;23(5):508-14. doi: 10.1590/1678-775720140049. Epub 2015 Jul 21.
The aim of this study was to evaluate the subcutaneous tissue response in rats and the antimicrobial activity of intracanal calcium hydroxide dressings mixed with different substances against E. faecalis. Fifty four rats were divided into three experimental groups according to the vehicle in the calcium hydroxide treatment: 0.4% chlorohexidine in propylene glycol (PG),Casearia sylvestris Sw in PG and calcium hydroxide+PG (control group). The pastes were placed into polyethylene tubes and implanted into the subcutaneous tissue. After 7, 14 and 30 days, the samples were processed and histologically evaluated (hematoxylin and eosin). The tissue surface in contact with the material was analyzed, and the quantitative analysis determined the volume density occupied by the inflammatory infiltrate (giant cells, polymorphonuclear cells and mononuclear cells), fibroblasts, collagen fibers and blood vessels. For the antimicrobial analysis, 20 dentin blocks infected with E. faecalis were treated with calcium hydroxide pastes in different vehicles; 0.4% chlorhexidine in PG, PG, extract from Casearia sylvestris Sw in PG and a positive control (infection and without medication) for 7 days. The efficiency of the pastes was evaluated by the live/dead technique and confocal microscopy. The results showed that 0.4% chlorhexidine induced a higher inflammatory response than the other groups. The Casearia sylvestris Sw extract showed satisfactory results in relation to the intensity of the inflammatory response. In the microbiological test, there were no statistical differences between the evaluated intracanal dressings and the percentage of bacterial viability was between 33 and 42%. The control group showed an 86% viability. Antimicrobial components such as chlorhexidine or Casearia sylvestris Sw did not improve the antimicrobial activity against E. faecalis in comparison to the calcium hydroxide+PG treatment. In addition, the incorporation of chlorhexidine in the calcium hydroxide paste promoted the highest inflammatory response.
本研究的目的是评估大鼠皮下组织的反应，以及与不同物质混合的根管内氢氧化钙敷料对粪肠球菌的抗菌活性。根据氢氧化钙治疗中使用的赋形剂，将54只大鼠分为三个实验组：丙二醇（PG）中含0.4%氯己定、PG中含西番莲提取物以及氢氧化钙+PG（对照组）。将糊剂放入聚乙烯管中并植入皮下组织。在7天、14天和30天后，对样本进行处理并进行组织学评估（苏木精和伊红染色）。分析与材料接触的组织表面，并通过定量分析确定炎症浸润（巨细胞、多形核细胞和单核细胞）、成纤维细胞、胶原纤维和血管所占的体积密度。对于抗菌分析，用不同赋形剂的氢氧化钙糊剂处理20个感染粪肠球菌的牙本质块；PG中含0.4%氯己定、PG、PG中含西番莲提取物以及阳性对照（感染且未用药），持续7天。通过活/死技术和共聚焦显微镜评估糊剂的效果。结果表明，0.4%氯己定比其他组诱导了更高的炎症反应。西番莲提取物在炎症反应强度方面显示出令人满意的结果。在微生物学测试中，评估的根管内敷料之间没有统计学差异，细菌存活率在33%至42%之间。对照组显示存活率为86%。与氢氧化钙+PG治疗相比，氯己定或西番莲等抗菌成分并未提高对粪肠球菌的抗菌活性。此外，在氢氧化钙糊剂中加入氯己定会引发最高的炎症反应。
