Takahashi Masayuki, Saito Atsushi, Jimbo Yasuhiko, Nakasono Satoshi
Environmental Science Research Laboratory, Central Research Institute of Electric Power Industry (CRIEPI).
J Toxicol Sci. 2017;42(2):223-231. doi: 10.2131/jts.42.223.
Although cardiac activity is known to differ between species in many respects, most evaluations of the cardiac effects of low-frequency electric and magnetic fields, which have a stimulant effect on electrically activated cells, have been performed in non-human experimental animals and cells, and the effects in humans have been assessed using theoretical models. In recent years, it has been verified that human cardiomyocytes differentiated from human induced pluripotent stem cells (hiPS-CM) are useful for evaluating human responses to various cardioactive compounds. In this study, we applied hiPSCMs for the first time to evaluate the human cardiac effects of power-frequency magnetic fields (MFs). After preparation of hiPS-CMs, we subjected a hiPS-CM monolayer formed on a multi-electrode array to short-term exposure to a 50 Hz MF at 400 mT with recording of the extracellular field potentials. The field potential duration of the hiPS-CMs did not differ significantly pre- and post-exposure, indicating that under these conditions, exposure to a 50 Hz MF at 400 mT does not affect the electrical activity of hiPSCMs.
虽然已知心脏活动在许多方面因物种而异，但大多数对低频电场和磁场心脏效应的评估（低频电场和磁场对电激活细胞有刺激作用）都是在非人类实验动物和细胞中进行的，而对人类的影响则是使用理论模型进行评估的。近年来，已证实从人诱导多能干细胞（hiPS-CM）分化而来的人类心肌细胞可用于评估人类对各种心脏活性化合物的反应。在本研究中，我们首次应用hiPSCM来评估工频磁场（MF）对人类心脏的影响。制备hiPS-CM后，我们将在多电极阵列上形成的hiPS-CM单层置于400 mT的50 Hz MF中进行短期暴露，并记录细胞外场电位。hiPS-CM的场电位持续时间在暴露前后没有显著差异，这表明在这些条件下，暴露于400 mT的50 Hz MF不会影响hiPSCM的电活动。
