Snodgrass R, Keller G
Basel Institute for Immunology, Switzerland.
EMBO J. 1987 Dec 20;6(13):3955-60. doi: 10.1002/j.1460-2075.1987.tb02737.x.
The clonal make-up of the haematopoietic system of mice reconstituted with retrovirus-infected bone marrow cells was analysed at two different points in time following reconstitution. We have found that under these conditions, the haematopoietic system consists of clones that persist throughout the 5 month course of the experiment as well as those which undergo temporal changes. The various changes that we have observed included the appearance of a new clone(s) in all lineages, the loss of a clone from some lineages and the shift in the appearance of a clone from one lineage to another. In addition, we provide evidence which suggests that the clonal make-up of the thymus changes with time; early after reconstitution it consists of many clones, whereas at the later time-points it contains a limited number of predominant clones. These studies document the dramatic clonal changes which occur within the various lineages for a long time following reconstitution and highlight the difficulty in demonstrating lineage-specific stem cells.
在利用逆转录病毒感染的骨髓细胞重建小鼠造血系统后，于重建后的两个不同时间点分析了其造血系统的克隆组成。我们发现，在这些条件下，造血系统由在整个5个月实验过程中持续存在的克隆以及经历暂时变化的克隆组成。我们观察到的各种变化包括所有谱系中出现新的克隆、某些谱系中一个克隆的消失以及一个克隆从一个谱系到另一个谱系的出现转移。此外，我们提供的证据表明胸腺的克隆组成随时间变化；重建后早期它由许多克隆组成，而在后期时间点它包含数量有限的优势克隆。这些研究记录了重建后很长一段时间内各个谱系中发生的显著克隆变化，并突出了证明谱系特异性干细胞的困难。
