Witke W, Nellen W, Noegel A
Max-Planck-Institut für Biochemie, Martinsried, FRG.
EMBO J. 1987 Dec 20;6(13):4143-8. doi: 10.1002/j.1460-2075.1987.tb02760.x.
Mutation of the alpha-actinin gene in Dictyostelium has been achieved by transforming cells with the Dictyostelium transformation vector pDNeoII containing a 1.2 kb fragment of the alpha-actinin gene. Transformants deficient in alpha-actinin, an actin-binding protein, produced an altered mRNA that lacked the 3' portion of the coding region. The defect in alpha-actinin production was not due to integration of the vector within the gene, but was apparently caused by errors produced during homologous recombination between the introduced alpha-actinin sequence and its complementary sequence in the coding region of the endogenous gene.
通过用含有α-肌动蛋白基因1.2 kb片段的盘基网柄菌转化载体pDNeoII转化细胞，实现了盘基网柄菌中α-肌动蛋白基因的突变。缺乏肌动蛋白结合蛋白α-肌动蛋白的转化体产生了一种改变的mRNA，该mRNA缺少编码区的3'部分。α-肌动蛋白产生缺陷并非由于载体整合到基因内部，而是显然由导入的α-肌动蛋白序列与其在内源基因编码区的互补序列之间同源重组过程中产生的错误所导致。
