Levesque Mitchell P, Cheng Phil F, Raaijmakers Marieke I G, Saltari Annalisa, Dummer Reinhard
Department of Dermatology, University of Zurich, University of Zurich Hospital, Gloriastrasse 31, CH-8091, Zurich, Switzerland.
Cancer Metastasis Rev. 2017 Mar;36(1):7-21. doi: 10.1007/s10555-017-9658-0.
Progress in understanding and treating metastatic melanoma is the result of decades of basic and translational research as well as the development of better in vitro tools for modeling the disease. Here, we review the latest therapeutic options for metastatic melanoma and the known genetic and non-genetic mechanisms of resistance to these therapies, as well as the in vitro toolbox that has provided the greatest insights into melanoma progression. These include next-generation sequencing technologies and more complex 2D and 3D cell culture models to functionally test the data generated by genomics approaches. The combination of hypothesis generating and hypothesis testing paradigms reviewed here will be the foundation for the next phase of metastatic melanoma therapies in the coming years.
对转移性黑色素瘤认识和治疗方面的进展，是数十年基础研究与转化研究以及用于模拟该疾病的更好体外工具发展的结果。在此，我们综述转移性黑色素瘤的最新治疗选择、对这些疗法产生耐药性的已知遗传和非遗传机制，以及为黑色素瘤进展提供了最深入见解的体外工具集。这些工具包括下一代测序技术以及更复杂的二维和三维细胞培养模型，用于对基因组学方法产生的数据进行功能测试。本文所综述的假设生成与假设检验范式的结合，将成为未来几年转移性黑色素瘤治疗下一阶段的基础。
