Chung Ill-Min, Rekha Kaliyaperumal, Rajakumar Govindasamy, Thiruvengadam Muthu
Department of Applied Bioscience, College of Life and Environmental Sciences, Konkuk University , Seoul - 143 701 , Republic of Korea.
Department of Environmental and Herbal Science, Tamil University , Thanjavur 613005, Tamil Nadu , India.
Acta Biol Hung. 2017 Mar;68(1):88-100. doi: 10.1556/018.68.2017.1.8.
In vitro cell suspension culture was established for the production of commercially valuable phytochemicals in Momordica dioica. The influence of elicitors in jasmonic acid (JA) and salicylic acid (SA) increased their effect on phytochemical production and biomass accumulation in M. dioica. The results indicate that compared with non-elicited cultures, JA- and SA-elicited cell suspension cultures had significantly enhanced phenolic, flavonoid, and carotenoid production, as well as antioxidant, antimicrobial, and antiproliferative activities. Furthermore, elicited cultures produced 22 phenolic compounds, such as flavonols, hydroxycinnamic acids, and hydroxybenzoic acids. Greater biomass production, phytochemical accumulation, and biological activity occurred in JA- than in SA-elicited cell cultures. This study is the first to successfully establish M. dioica cell suspension cultures for the production of phenolic compounds and carotenoids, as well as for biomass accumulation.
为了生产具有商业价值的苦瓜植物化学物质，建立了体外细胞悬浮培养体系。茉莉酸（JA）和水杨酸（SA）中的诱导子对苦瓜植物化学物质的产生和生物量积累的影响增强。结果表明，与未诱导的培养物相比，JA和SA诱导的细胞悬浮培养物中酚类、黄酮类和类胡萝卜素的产量显著提高，抗氧化、抗菌和抗增殖活性也显著增强。此外，诱导培养物产生了22种酚类化合物，如黄酮醇、羟基肉桂酸和羟基苯甲酸。与SA诱导的细胞培养物相比，JA诱导的细胞培养物中生物量产量更高、植物化学物质积累更多且生物活性更强。本研究首次成功建立了用于生产酚类化合物和类胡萝卜素以及积累生物量的苦瓜细胞悬浮培养体系。
