Thiruvengadam Muthu, Rekha Kaliyaperumal, Rajakumar Govindasamy, Lee Taek-Jun, Kim Seung-Hyun, Chung Ill-Min
Department of Applied Bioscience, College of Life and Environmental Sciences, Konkuk University, Seoul 143 701, Korea.
Int J Mol Sci. 2016 Nov 16;17(11):1912. doi: 10.3390/ijms17111912.
Anthraquinones (AQs) and phenolic compounds are important phytochemicals that are biosynthesized in cell suspension cultures of . We wanted to optimize the effects of plant growth regulators (PGRs), media, sucrose, l-glutamine, jasmonic acid (JA), and salicylic acid (SA) for the production of phytochemicals and biomass accumulation in a cell suspension culture of . The medium containing Murashige and Skoog (MS) salts and 4% sucrose supplemented with 1 mg/L 2,4-dichlorophenoxyacetic acid, 0.5 mg/L thidiazuron, and 100 µM l-glutamine at 28 days of cell suspension culture was suitable for biomass accumulation and AQ production. Maximum biomass accumulation (12.5 and 12.35 g fresh mass (FM); 3 and 2.93 g dry mass (DM)) and AQ production (emodin 295.20 and 282 mg/g DM; physcion 421.55 and 410.25 mg/g DM) were observed using 100 µM JA and SA, respectively. JA- and SA-elicited cell cultures showed several-fold higher biomass accumulation and AQ production than the control cell cultures. Furthermore, the cell suspension cultures effectively produced 23 phenolic compounds, such as flavonols and hydroxycinnamic and hydroxybenzoic acid derivatives. PGR-, JA-, and SA-elicited cell cultures produced a higher amount of AQs and phenolic compounds. Because of these metabolic changes, the antioxidant, antimicrobial, and anticancer activities were high in the PGR-, JA-, and SA-elicited cell cultures. The results showed that the elicitors (JA and SA) induced the enhancement of biomass accumulation and phytochemical (AQs and phenolic compounds) production as well as biological activities in the cell suspension cultures of . This optimized protocol can be developed for large-scale biomass accumulation and production of phytochemicals (AQs and phenolic compounds) from cell suspension cultures, and the phytochemicals can be used for various biological activities.
蒽醌(AQs)和酚类化合物是重要的植物化学物质,它们在……的细胞悬浮培养物中生物合成。我们希望优化植物生长调节剂(PGRs)、培养基、蔗糖、L-谷氨酰胺、茉莉酸(JA)和水杨酸(SA)对……细胞悬浮培养物中植物化学物质生产和生物量积累的影响。含有Murashige和Skoog(MS)盐以及4%蔗糖,并补充1 mg/L 2,4-二氯苯氧乙酸、0.5 mg/L噻苯隆和100 µM L-谷氨酰胺的培养基,在细胞悬浮培养28天时,适合生物量积累和AQ生产。分别使用100 µM JA和SA时,观察到最大生物量积累(12.5和12.35 g鲜重(FM);3和2.93 g干重(DM))和AQ生产(大黄素295.20和282 mg/g DM;大黄酚421.55和410.25 mg/g DM)。JA和SA诱导的细胞培养物的生物量积累和AQ生产比对照细胞培养物高出几倍。此外,细胞悬浮培养物有效地产生了23种酚类化合物,如黄酮醇以及羟基肉桂酸和羟基苯甲酸衍生物。PGR、JA和SA诱导的细胞培养物产生了更高量的AQs和酚类化合物。由于这些代谢变化,PGR、JA和SA诱导的细胞培养物中的抗氧化、抗菌和抗癌活性较高。结果表明,诱导剂(JA和SA)在……的细胞悬浮培养物中诱导了生物量积累、植物化学物质(AQs和酚类化合物)生产以及生物活性的增强。这种优化方案可用于大规模生物量积累以及从细胞悬浮培养物中生产植物化学物质(AQs和酚类化合物),并且这些植物化学物质可用于各种生物活性。