Department of Horticultural Science, Division of Animal, Horticulture and Food Sciences, Chungbuk National University, Cheongju, 28644, Republic of Korea.
Hankookshinyak Pharmaceutical Co. Ltd., Nonsan Chungnam, 28433, Republic of Korea.
Appl Microbiol Biotechnol. 2018 Jan;102(1):199-209. doi: 10.1007/s00253-017-8629-2. Epub 2017 Nov 14.
We examined the effects of abiotic (methyl jasmonate [MeJA] and salicylic acid [SA]) and biotic (yeast extract and chitosan) elicitors for improvement of bioactive compounds production on adventitious root cultures in Polygonum multiflorum. The application of yeast extract resulted in significantly (p ≤ 0.05) higher dry root biomass (9.98 g/L) and relative growth rate versus the control. Cultures treated with abiotic elicitors showed higher percentage of dry weight than the other samples. Low concentrations of all elicitors (50 μM MeJA and SA, and 50 mg/L yeast extract) improved secondary metabolite production except for chitosan, whose performance was worse than that of the control. HPLC analysis of various bioactive compounds revealed significantly higher elicitation efficiency for MeJA than for the other treatments, with an approximately 2-fold increase in root dry weight (22.08 mg/g DW) under 50 μM MeJA treatment versus the control (10.35 mg/g DW). We also investigated the feasibility of scaling up the production process by comparing shake flask cultures with 3- and 5-L balloon type bubble bioreactors (BTBB) using 50 μM MeJA as an elicitor. Growth and metabolite accumulation increased in BTBB compared with shake flask cultures. We detected a non-significant difference in biomass productivity between 3 and 5-L BTBB, but the efficiency of bioactive compound accumulation decreased with increasing volume. These findings will be useful for developing a pilot-scale P. multiflorum adventitious root cultivation process for high biomass and bioactive compound production to meet the demands for natural ingredients by the pharmaceutical and cosmetic industries without affecting the natural habitat of this plant.
我们研究了非生物（茉莉酸甲酯[MeJA]和水杨酸[SA]）和生物（酵母提取物和壳聚糖）诱导子对何首乌不定根培养中生物活性化合物生产的影响。与对照相比，酵母提取物的应用导致干根生物量（9.98 g/L）和相对生长率显著提高（p≤0.05）。用生物诱导子处理的培养物的干重百分比高于其他样品。除壳聚糖外，所有诱导剂（50 μM MeJA 和 SA，50 mg/L 酵母提取物）的低浓度均提高了次生代谢产物的产量，而壳聚糖的表现则不如对照。各种生物活性化合物的 HPLC 分析表明，MeJA 的诱导效率明显高于其他处理，在 50 μM MeJA 处理下，根干重增加了约 2 倍（22.08 mg/g DW），而对照（10.35 mg/g DW）。我们还通过比较摇瓶培养和 3 升和 5 升气球式泡生物反应器（BTBB），使用 50 μM MeJA 作为诱导剂，研究了放大生产过程的可行性。与摇瓶培养相比，BTBB 中的生长和代谢产物积累增加。我们在 3 升和 5 升 BTBB 之间检测到生物量生产力没有显著差异，但随着体积的增加，生物活性化合物积累的效率降低。这些发现将有助于开发何首乌不定根培养的中试规模生产工艺，以满足制药和化妆品行业对天然成分的需求，同时又不影响该植物的自然栖息地。
