毒蕈碱受体介导的胚胎鸡心脏细胞内钙离子动员
Muscarinic receptor-mediated intracellular Ca2+ mobilization in embryonic chick heart cells.
作者信息
Schmidt H, Oettling G, Drews U
机构信息
Anatomisches Institut der Universität Tübingen, FRG.
出版信息
FEBS Lett. 1988 Mar 28;230(1-2):35-7. doi: 10.1016/0014-5793(88)80636-7.
Activation of muscarinic receptors of heart cells elevates the intracellular Ca2+ concentration. The increase is considered to be due to influx of extracellular Ca2+. We show that intracellular Ca2+ mobilization is involved. Cell suspensions prepared from hearts of 6-day-old chick embryos were loaded with the fluorescent Ca2+ chelator chlortetracycline. Muscarinic stimulation induces a dose-dependent fluorescence decrease (ED50 = 2.6 x 10(-6) M) indicating intracellular Ca2+ mobilization.
心脏细胞毒蕈碱受体的激活会提高细胞内钙离子浓度。这种增加被认为是由于细胞外钙离子的流入所致。我们发现细胞内钙离子动员也参与其中。从6日龄鸡胚心脏制备的细胞悬液用荧光钙离子螯合剂氯四环素进行负载。毒蕈碱刺激会诱导剂量依赖性的荧光降低（半数有效剂量=2.6×10⁻⁶ M），表明细胞内钙离子动员。
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