鸡胚中钙离子动员毒蕈碱系统的表达与形态发生相关。

Expression of the Ca2+ mobilizing muscarinic system in the chick embryo correlates with morphogenesis.

作者信息

Oettling G, Schmidt H, Show-Klett A, Drews U

机构信息

Institute of Anatomy, University of Tübingen, F.R.G.

出版信息

Cell Differ. 1988 Mar;23(1-2):77-86. doi: 10.1016/0045-6039(88)90039-5.

DOI:10.1016/0045-6039(88)90039-5

PMID:3370681

Abstract

We describe muscarinic receptors and intracellular Ca2+ mobilization after cholinergic stimulation in cell suspensions prepared from chick embryos between day 2 (stage 12/13) and day 13 (stage 40) of development. Cell suspensions are prepared from whole chick embryos and from embryonic hearts, heads or brains, limb buds, and trunks. Muscarinic receptors are measured using [3H]quinuclidinylbenzilate as specific ligand. Intracellular Ca2+ mobilization is determined by changes of chlorotetracycline fluorescence. (1) Considerable amounts of muscarinic receptors are found in all parts of the embryo and at all stages tested. (2) The intracellular Ca2+ response after stimulation by muscarinic agonist shows a peak at day 3-4 (stage 23). (3) The pharmacological profile of the Ca2+ response remains constant during embryonic development and differs from the profiles of most adult systems. (4) The 'embryonic muscarinic system' is uniformly expressed in cells from neural and non-neural tissues. It appears and disappears independently of innervation.

摘要

我们描述了在发育第2天（第12/13阶段）至第13天（第40阶段）的鸡胚制备的细胞悬液中，胆碱能刺激后的毒蕈碱受体和细胞内Ca2+动员情况。细胞悬液取自整个鸡胚以及胚胎心脏、头部或大脑、肢芽和躯干。使用[3H]喹核酯作为特异性配体来测量毒蕈碱受体。通过氯四环素荧光变化来确定细胞内Ca2+动员情况。（1）在胚胎的所有部位以及所有测试阶段均发现了大量的毒蕈碱受体。（2）毒蕈碱激动剂刺激后的细胞内Ca2+反应在第3 - 4天（第23阶段）出现峰值。（3）Ca2+反应的药理学特征在胚胎发育过程中保持不变，且与大多数成年系统的特征不同。（4）“胚胎毒蕈碱系统”在神经组织和非神经组织的细胞中均有一致表达。它的出现和消失与神经支配无关。