Department of Pathology, Zhongshan Hospital, Fudan University, Shanghai 200032, P. R. China.
Department of Thoracic surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, P. R. China.
Sci Rep. 2017 Mar 21;7:44823. doi: 10.1038/srep44823.
We undertook a study of tumour infiltrating lymphocytes (TILs) in a large and relatively homogeneous group of patients with completely resected esophageal squamous cell carcinoma (ESCC). Hematoxylin and eosin-stained sections of 235 ESCC tumours were evaluated for density of TILs in intratumoural (iTIL) and stromal compartments (sTIL). Foxp3+, CD4+, and CD8+ T cells in tumoural and stromal areas were evaluated by immunohistochemistry. Of the 235 tumours, high sTIL (>10%), and iTIL (>10%) were observed in 101 (43.0%) and 98 (41.7%), respectively. The median follow-up period was 36.0 months (95% CI 29.929-42.071). Univariate analysis revealed that sTIL (>10%), iTIL (>20%), vessels involvement, lymph node metastasis, and clinical stage were significantly associated with postoperative outcome. In multivariate analysis, high sTIL (HR: 0.664, P = 0.019 for Disease free survival; HR: 0.608, P = 0.005 for Overall survival) was identified as independent better prognostic factor. Further analysis, sTIL was identified as independently prognostic factor in Stage III-IVa disease, which was not found in Stage I-II disease. Our study demonstrated that sTIL was associated with better ESCC patients' survival, especially in Stage III-IVa disease. Assessment of sTIL could be useful to discriminate biological behavior for ESCC patients.
我们对 235 例完全切除的食管鳞状细胞癌（ESCC）患者的肿瘤浸润淋巴细胞（TILs）进行了研究。对 235 例 ESCC 肿瘤的苏木精和伊红染色切片进行了肿瘤内（iTIL）和基质区（sTIL）TIL 密度评估。采用免疫组织化学法评估肿瘤和基质区的 Foxp3+、CD4+和 CD8+T 细胞。在 235 例肿瘤中，分别有 101 例（43.0%）和 98 例（41.7%）观察到高 sTIL（>10%）和 iTIL（>10%）。中位随访时间为 36.0 个月（95%CI 29.929-42.071）。单因素分析显示，sTIL（>10%）、iTIL（>20%）、血管侵犯、淋巴结转移和临床分期与术后结果显著相关。多因素分析显示，高 sTIL（无病生存率的 HR：0.664，P=0.019；总生存率的 HR：0.608，P=0.005）是独立的预后较好的因素。进一步分析表明，sTIL 是 III-IVa 期疾病的独立预后因素，但在 I-II 期疾病中未发现。本研究表明，sTIL 与 ESCC 患者的生存相关，尤其是在 III-IVa 期疾病中。sTIL 的评估可能有助于区分 ESCC 患者的生物学行为。
