Department of Medicine, The University of Hong Kong, Hong Kong.
Centre for Genomic Sciences, The University of Hong Kong, Hong Kong.
Sci Rep. 2017 Mar 21;7:44935. doi: 10.1038/srep44935.
The role of bacteria other than Helicobacter pylori (HP) in the stomach remains elusive. We characterized the gastric microbiota in individuals with different histological stages of gastric carcinogenesis and after receiving HP eradication therapy. Endoscopic gastric biopsies were obtained from subjects with HP gastritis, gastric intestinal metaplasia (IM), gastric cancer (GC) and HP negative controls. Gastric microbiota was characterized by Illumina MiSeq platform targeting the 16 S rDNA. Apart from dominant H. pylori, we observed other Proteobacteria including Haemophilus, Serratia, Neisseria and Stenotrophomonas as the major components of the human gastric microbiota. Although samples were largely converged according to the relative abundance of HP, a clear separation of GC and other samples was recovered. Whilst there was a strong inverse association between HP relative abundance and bacterial diversity, this association was weak in GC samples which tended to have lower bacterial diversity compared with other samples with similar HP levels. Eradication of HP resulted in an increase in bacterial diversity and restoration of the relative abundance of other bacteria to levels similar to individuals without HP. In conclusion, HP colonization results in alterations of gastric microbiota and reduction in bacterial diversity, which could be restored by antibiotic treatment.
除幽门螺杆菌（HP）以外的细菌在胃中的作用仍不明确。我们对不同胃组织学癌变阶段和接受 HP 根除治疗后的个体的胃微生物群进行了特征描述。从 HP 胃炎、胃肠化生（IM）、胃癌（GC）和 HP 阴性对照组患者中获取内镜胃活检。通过靶向 16S rDNA 的 Illumina MiSeq 平台对胃微生物群进行了特征描述。除了优势的幽门螺杆菌，我们还观察到其他变形菌门，包括嗜血杆菌、沙雷氏菌、奈瑟菌和寡养单胞菌，是人类胃微生物群的主要组成部分。尽管根据 HP 的相对丰度，样本有很大的趋同，但 GC 和其他样本之间的明显分离得以恢复。虽然 HP 相对丰度与细菌多样性之间存在很强的负相关，但这种相关性在 GC 样本中较弱，与其他具有相似 HP 水平的样本相比，GC 样本的细菌多样性往往较低。HP 的根除导致细菌多样性增加，其他细菌的相对丰度恢复到类似于没有 HP 的个体的水平。总之，HP 定植导致胃微生物群发生改变，细菌多样性减少，抗生素治疗可使其恢复。
