Ahn Meejung, Won Seunghwan, Kang Bongjo, Gong Po, Yoo Eunho, Dharaneedharan Subramanian, Jang Yeounghwan
School of Medicine, Jeju National University, Jeju 63243, Republic of Korea.
Ocean and Fisheries Research Institute, Jeju Special Self-Governing Province, Pyoseon-myeon, Segwipo-si, Jeju 63629, Republic of Korea.
Parasite. 2017;24:11. doi: 10.1051/parasite/2017012. Epub 2017 Mar 21.
Kudoa septempunctata (Myxozoa: Multivalvulida) myxospores infect the trunk muscles of olive flounder (Paralichthys olivaceus). In this study, two popular commercially formulated anti-coccidial drugs (amprolium hydrochloride and toltrazuril) were serially diluted and incubated with purified mature Kudoa septempunctata myxospores. The viability of K. septempunctata spores was determined after a 2-day incubation followed by Hoechst 33342 and propidium iodide staining, and scanning electron microscopy. Amprolium hydrochloride significantly decreased spore viability (18% of control) at a concentration of 920 μg/mL, whereas toltrazuril showed almost no effect (83% of control). Viability of the control (untreated spores) was 90%. In vivo studies are required to confirm the efficacy of amprolium hydrochloride in fish infected with K. septempunctata myxospores on their growth and immune system performance.
七鳃鳗库道虫（粘孢子虫纲：多瓣虫目）的粘孢子感染牙鲆（Paralichthys olivaceus）的躯干肌肉。在本研究中，将两种常用的市售抗球虫药物（盐酸氨丙啉和托曲珠利）进行系列稀释，并与纯化的成熟七鳃鳗库道虫粘孢子一起孵育。在2天孵育后，通过Hoechst 33342和碘化丙啶染色以及扫描电子显微镜确定七鳃鳗库道虫孢子的活力。盐酸氨丙啉在浓度为920μg/mL时显著降低了孢子活力（为对照的18%），而托曲珠利几乎没有效果（为对照的83%）。对照（未处理的孢子）的活力为90%。需要进行体内研究以确认盐酸氨丙啉对感染七鳃鳗库道虫粘孢子的鱼的生长和免疫系统性能的疗效。
