Trevisan Chiara, Montillo Marta, Prandi Alberto, Mkupasi Ernatus M, Ngowi Helena A, Johansen Maria V
Department of Veterinary and Animal Sciences, University of Copenhagen, Dyrlægevej 100, 1870 Frederiksberg C, Denmark.
Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Via Sondrio 2/A, 33100 Udine, Italy.
Gen Comp Endocrinol. 2017 May 15;246:23-28. doi: 10.1016/j.ygcen.2017.03.007. Epub 2017 Mar 16.
The aim of this study was to measure hair cortisol and dehydroepiandrosterone (DHEA) concentrations in naturally Taenia solium infected and non-infected control pigs and assess the effect of an environmental change on the aforementioned parameters. Three hair patches were obtained from 13 T. solium infected and 15 non-infected controls sows, respectively corresponding to 3 time points (prior to, at and approximately two weeks after arrival at the research facility). Cortisol and DHEA were extracted using methanol and analysed by radio immune assay. Mean hair cortisol concentrations were significantly lower (p<0.001) in T. solium infected (4.7±3.0pg/mg) compared to control pigs (9.0±3.7pg/mg) prior to arrival at the research facility, however no significant difference was observed between the two groups at arrival and after approximately two weeks. Similar patterns were also observed for DHEA concentrations (infected pigs 253.9±82.3pg/mg, control pigs 387.7±116.4pg/mg) (p<0.001). Results showed that lean animals had significantly higher cortisol concentrations in both groups, infected and controls pigs, while DHEA was not significantly different between lean and normal animals. Results of this study have shown that an environmental change could have an effect on pigs' hormonal levels suggesting an undergoing adaptation process. After the pigs were kept under the same conditions, fed and watered ad libitum, no significant differences were observed between the groups, but a drop in DHEA concentrations was observed in all the pigs. Weight however had an effect on cortisol levels as lean animals had significantly higher cortisol concentrations in both groups, compared to normal pigs.
本研究的目的是测量自然感染猪带绦虫和未感染的对照猪毛发中的皮质醇和脱氢表雄酮（DHEA）浓度，并评估环境变化对上述参数的影响。分别从13头感染猪带绦虫的母猪和15头未感染的对照母猪身上获取三块毛发样本，分别对应3个时间点（到达研究设施之前、到达时以及到达后约两周）。使用甲醇提取皮质醇和DHEA，并通过放射免疫分析法进行分析。在到达研究设施之前，感染猪带绦虫的猪（4.7±3.0pg/mg）的平均毛发皮质醇浓度显著低于对照猪（9.0±3.7pg/mg）（p<0.001），然而在到达时和大约两周后，两组之间未观察到显著差异。DHEA浓度也观察到类似模式（感染猪253.9±82.3pg/mg，对照猪387.7±116.4pg/mg）（p<0.001）。结果表明，在感染组和对照组中，瘦猪的皮质醇浓度均显著较高，而瘦猪和正常猪之间的DHEA没有显著差异。本研究结果表明，环境变化可能会影响猪的激素水平，表明其正在经历适应过程。在猪在相同条件下自由采食和饮水后，两组之间未观察到显著差异，但所有猪的DHEA浓度均有所下降。然而，体重对皮质醇水平有影响，因为与正常猪相比，两组中的瘦猪皮质醇浓度均显著较高。
