Ortiz S, Osorio G, Solari A
Programa de Biología Celular y Molecular, ICBM, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
Programa de Microbiología, ICBM, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
Infect Genet Evol. 2017 Jul;51:104-107. doi: 10.1016/j.meegid.2017.03.017. Epub 2017 Mar 16.
The taxon Trypanosoma cruzi, causative agent of Chagas disease, is composed of several discrete typing units (DTUs) named TcI-TcVI, and Tcbat. The history of the taxon T. cruzi is known, even though several controversial aspects remain as the relationships between TcIII and TcIV. We analyzed cloned T. cruzi stocks pertaining to the seven DTUs by filter hybridization tests of PCR amplicons from minicircle variable regions and kinetoplast DNA probes. Minicircle DNA blots from the cloned stocks and filter hybridization with one TcI, one TcII, one TcV, one TcVI, three TcIII, one TcIV from North America and one TcIV kinetoplast DNA probes from South America revealed minicircle variable region cross-reaction in some T. cruzi DTUs probed. TcIII was heterogeneous in minicircle class composition, even though two TcIII probes revealed that a small fraction of minicircles cross-hybridized with the minicircles from the TcIII, TcV and TcVI DTUs. The minicircles of TcIV from North America cross-reacted only with TcIV from North America but not with TcIV stocks from Brazil and Bolivia. The results on minicircle cross-hybridizations are discussed in the context of RNA editing, mitochondrial function in T. cruzi DTUs.
克氏锥虫是恰加斯病的病原体，它由几个离散型单元（DTUs）组成，分别命名为TcI - TcVI和Tcbat。尽管克氏锥虫分类单元的历史是已知的，但仍存在一些有争议的方面，如TcIII和TcIV之间的关系。我们通过对来自小环可变区的PCR扩增产物与动基体DNA探针进行滤膜杂交试验，分析了属于七个DTUs的克隆克氏锥虫菌株。来自克隆菌株的小环DNA印迹以及与一个TcI、一个TcII、一个TcV、一个TcVI、三个TcIII、一个来自北美的TcIV和一个来自南美的TcIV动基体DNA探针的滤膜杂交显示，在一些被检测的克氏锥虫DTUs中存在小环可变区交叉反应。TcIII在小环类组成上是异质的，尽管两个TcIII探针显示一小部分小环与来自TcIII、TcV和TcVI DTUs的小环交叉杂交。来自北美的TcIV小环仅与来自北美的TcIV交叉反应，而不与来自巴西和玻利维亚的TcIV菌株交叉反应。本文在RNA编辑、克氏锥虫DTUs线粒体功能的背景下讨论了小环交叉杂交的结果。
