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人类致病寄生虫的线粒体基因组学（）。 （你提供的原文括号部分内容缺失，请补充完整以便准确翻译。）

Mitochondrial genomics of human pathogenic parasite () .

作者信息

Urrea Daniel Alfonso, Triana-Chavez Omar, Alzate Juan F

机构信息

Laboratorio de Investigaciones en Parasitología Tropical (LIPT), Departamento de Biología, Facultad de Ciencias, Universidad del Tolima, Ibague, Tolima, Colombia.

Grupo Biología y Control de Enfermedades Infecciosas (BCEI), Universidad de Antioquia, Medellín, Antioquia, Colombia.

出版信息

PeerJ. 2019 Jul 2;7:e7235. doi: 10.7717/peerj.7235. eCollection 2019.

DOI:10.7717/peerj.7235
PMID:31304069
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6611448/
Abstract

BACKGROUND

The human parasite is one of the pathogenic species responsible for cutaneous leishmaniasis in Central and South America. Despite its importance in molecular parasitology, its mitochondrial genome, divided into minicircles and maxicircles, haven't been described so far.

METHODS

Using NGS-based sequencing (454 and ILLUMINA), and combining genome assembly and mapping strategies, we report the maxicircle kDNA annotated genome of , the first reference of this molecule for the subgenus . A comparative genomics approach is performed against other and species.

RESULTS

The results show synteny of mitochondrial genes of with other kinetoplastids. It was also possible to identify nucleotide variants within the coding regions of the maxicircle, shared among some of them and others specific to each strain. Furthermore, we compared the minicircles kDNA sequences of two strains and the results show that the conserved and divergent regions of the minicircles exhibit strain-specific associations.

摘要

背景

这种人类寄生虫是中南美洲皮肤利什曼病的致病物种之一。尽管其在分子寄生虫学中具有重要意义，但其线粒体基因组分为小环和大环，迄今为止尚未被描述。

方法

使用基于二代测序技术（454和ILLUMINA），并结合基因组组装和定位策略，我们报告了该寄生虫的大环动质体DNA注释基因组，这是该亚属该分子的首个参考基因组。对其他该属和种进行了比较基因组学分析。

结果

结果表明该寄生虫的线粒体基因与其他动质体具有共线性。还能够识别大环编码区域内的核苷酸变异，其中一些变异在部分菌株中共享，而其他变异则是每个菌株特有的。此外，我们比较了两个菌株的小环动质体DNA序列，结果表明小环的保守和发散区域呈现出菌株特异性关联。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e94/6611448/781a91ea43a2/peerj-07-7235-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e94/6611448/31336c4f9710/peerj-07-7235-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e94/6611448/bfd3e913ffd1/peerj-07-7235-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e94/6611448/20d12b5a59e0/peerj-07-7235-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e94/6611448/781a91ea43a2/peerj-07-7235-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e94/6611448/31336c4f9710/peerj-07-7235-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e94/6611448/bfd3e913ffd1/peerj-07-7235-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e94/6611448/20d12b5a59e0/peerj-07-7235-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e94/6611448/781a91ea43a2/peerj-07-7235-g004.jpg

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The kinetoplast DNA of the Australian trypanosome, Trypanosoma copemani, shares features with Trypanosoma cruzi and Trypanosoma lewisi.
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