Daniluk Jaroslaw, Daniluk Urszula, Rusak Malgorzata, Dabrowska Milena, Reszec Joanna, Garbowicz Magdalena, Huminska Kinga, Dabrowski Andrzej
Department of Gastroenterology and Internal Medicine, Medical University of Bialystok ul, M. Sklodowskiej-Curie 24a, 15-276 Bialystok, Poland.
Department of Pediatrics, Gastroenterology and Allergology, Medical University of Bialystok ul, J. Waszyngtona 17, 15-274 Bialystok, Poland.
Anaerobe. 2017 Oct;47:18-24. doi: 10.1016/j.anaerobe.2017.03.015. Epub 2017 Mar 18.
Antibiotics have many beneficial effects but their uncontrolled use may lead to increased risk of serious diseases in the future. Our hypothesis is that an early antibiotic exposition may affect immune system by altering gut microbiota. Therefore, the aim of the study was to determine the effect of penicillin treatment on gut microorganisms and immune system of mice.
21-days old C57BL6/J/cmdb male mice were treated with low-dose of penicillin (study group) or water only (control group) for 4 weeks. Tissue and stool samples for histology or microbiome assessment and peripheral blood for CBC and flow cytometry evaluation were collected.
We found high variability in microbiota composition at different taxonomic levels between littermate mice kept in the same conditions, independently of treatment regimen. Interestingly, low-dose of penicillin caused significant increase of Parabacteroides goldsteinii in stool and in colon tissue in comparison to control group (9.5% vs. 4.9%, p = 0.008 and 10.7% vs. 6.1%, p = 0.008, respectively). Moreover, mice treated with penicillin demonstrated significantly elevated percentage of B cells (median 10.5% vs 8.0%, p = 0.01) and decrease in the percentage of total CD4 cell (median 75.4% vs 82.5%, p = 0.0039) with subsequent changes among subsets - increased percentage of regulatory T cells (Treg), T helper 1 (Th1) and T helper 2 (Th2) cells.
Our study showed significant effect of penicillin on B and T cells in peripheral blood of young mice. This effect may be mediated through changes in gut microbiota represented by the expansion of Parabacteroides goldsteinii.
抗生素有诸多有益作用，但其无节制使用可能会在未来增加患严重疾病的风险。我们的假设是，早期接触抗生素可能通过改变肠道微生物群来影响免疫系统。因此，本研究的目的是确定青霉素治疗对小鼠肠道微生物和免疫系统的影响。
对21日龄的C57BL6/J/cmdb雄性小鼠，一组用低剂量青霉素治疗（研究组），另一组仅给予水（对照组），持续4周。收集用于组织学或微生物组评估的组织和粪便样本，以及用于全血细胞计数和流式细胞术评估的外周血。
我们发现，在相同条件下饲养的同窝小鼠之间，不同分类水平的微生物群组成存在很大差异，且与治疗方案无关。有趣的是，与对照组相比，低剂量青霉素使粪便和结肠组织中的戈氏副拟杆菌显著增加（分别为9.5%对4.9%，p = 0.008；10.7%对6.1%，p = 0.008）。此外，用青霉素治疗的小鼠B细胞百分比显著升高（中位数10.5%对8.0%，p = 0.01），总CD4细胞百分比降低（中位数75.4%对82.5%，p = 0.0039），随后各亚群发生变化——调节性T细胞（Treg）、辅助性T细胞1（Th1）和辅助性T细胞2（Th2）百分比增加。
我们的研究表明，青霉素对幼鼠外周血中的B细胞和T细胞有显著影响。这种影响可能是通过以戈氏副拟杆菌扩张为代表的肠道微生物群变化介导的。
