O'Banion M K, Sundberg J P, Reszka A A, Reichmann M E
Department of Microbiology, University of Illinois, Urbana 61801.
Intervirology. 1987;28(2):114-21. doi: 10.1159/000150004.
Cloned DNAs of 21 different papillomaviruses which naturally infect mammals and one bird papillomavirus were compared for relative homology by Southern blot hybridization. Blots were carried out under low (Tm-40 degrees), medium (Tm-33 degrees), and high (Tm-22 degrees) stringency conditions. At higher stringency, human papillomaviruses cross-hybridized with each other reflecting species-specific similarities. Bovine papillomavirus types 1, 2, 5, European elk papillomavirus, and deer papillomavirus also cross-hybridized at higher stringencies probably reflecting the association of these viruses with fibroblast-prolific lesions. The hybridization data presented here may be useful in future classification attempts. They are also useful as a guide in the selection of papillomavirus DNA probes for analysis of extracts from warts and tumors.
通过Southern印迹杂交比较了21种自然感染哺乳动物的不同乳头瘤病毒的克隆DNA和一种禽乳头瘤病毒的相对同源性。印迹在低（Tm - 40℃）、中（Tm - 33℃）和高（Tm - 22℃）严格条件下进行。在较高严格度下，人乳头瘤病毒彼此交叉杂交，反映了种属特异性相似性。牛乳头瘤病毒1型、2型、5型、欧洲马鹿乳头瘤病毒和鹿乳头瘤病毒在较高严格度下也交叉杂交，这可能反映了这些病毒与成纤维细胞增殖性病变的关联。这里呈现的杂交数据可能对未来的分类尝试有用。它们也可作为选择乳头瘤病毒DNA探针以分析疣和肿瘤提取物的指导。
