Campo M S, Coggins L W
J Gen Virol. 1982 Dec;63(2):255-64. doi: 10.1099/0022-1317-63-2-255.
The genomic DNAs of bovine papillomavirus (BPV) type 1, type 2 and type 4 were cloned in pAT153. BPV1 and BPV2 genomes were cloned using the single HindIII sites of the vector and virus DNAs, and BPV4 was cloned using the single BamHI sites. The orientation of the recombinant DNAs was established by restriction enzyme digestion, hybridization and heteroduplex analysis. The results showed that: (i) BPV1 and BPV2 DNAs are in register and are broadly homologous throughout most of their length when aligned at their single HindIII sites; (ii) depending on the degree of hybridization stringency used, the two DNAs show one major region and several minor regions of partial homology, mainly residing in the segment of the genomes believed to contain the structural genes; (iii) BPV4 DNA shares no homology with either BPV1 or BPV2 DNA.
牛乳头瘤病毒1型、2型和4型的基因组DNA被克隆到pAT153中。BPV1和BPV2基因组利用载体和病毒DNA的单一HindIII位点进行克隆，BPV4则利用单一BamHI位点进行克隆。通过限制性内切酶消化、杂交和异源双链分析确定重组DNA的方向。结果表明：（i）BPV1和BPV2 DNA在其单一HindIII位点对齐时是对齐的，并且在其大部分长度上具有广泛的同源性；（ii）根据所用杂交严谨度的不同，这两种DNA显示出一个主要同源区域和几个部分同源的次要区域，主要位于基因组中被认为包含结构基因的片段中；（iii）BPV4 DNA与BPV1或BPV2 DNA均无同源性。
