一种模拟10-23 DNA酶的52个核苷酸的DNA/2'-OMe-RNA寡聚物与底物形成复合物的合成、结晶及初步晶体学分析。

Synthesis, crystallization and preliminary crystallographic analysis of a 52-nucleotide DNA/2'-OMe-RNA oligomer mimicking 10-23 DNAzyme in the complex with a substrate.

作者信息

Dolot Rafał, Sobczak Milena, Mikołajczyk Barbara, Nawrot Barbara

机构信息

a Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences , Department of Bioorganic Chemistry , Łódź , Poland.

出版信息

Nucleosides Nucleotides Nucleic Acids. 2017 Apr 3;36(4):292-301. doi: 10.1080/15257770.2016.1276291. Epub 2017 Mar 21.

DOI:10.1080/15257770.2016.1276291

PMID:28323518

Abstract

A 52-nucleotide DNA/2'-OMe-RNA oligomer mimicking 10-23 DNAzyme in the complex with its substrate was synthesized, purified and crystallized by the hanging-drop method using 0.8 M sodium potassium tartrate as a precipitant. A data set to 1.21 Å resolution was collected from a monocrystal at 100 K using synchrotron radiation on a beamline BL14.1 at BESSY. The crystal belonged to the P2 group with unit-cell a = 49.42, b = 24.69, c = 50.23, β = 118.48.

摘要

合成了一种52个核苷酸的DNA/2'-O-甲基-RNA寡聚物，其模拟与底物形成复合物的10-23 DNA酶，通过悬滴法使用0.8 M酒石酸钠钾作为沉淀剂进行纯化和结晶。在100 K下，使用BESSY的BL14.1光束线上的同步辐射从单晶收集了分辨率为1.21 Å的数据集。该晶体属于P2空间群，晶胞参数为a = 49.42，b = 24.69，c = 50.23，β = 118.48。