Salisbury Phillip A, Fripp Yvonne J, Gurung Allison M, Williams Warren M
Faculty of Veterinary and Agricultural Sciences, University of Melbourne, Parkville, Victoria, Australia.
AgriBio, Centre for AgriBioscience, Department of Economic Development, Jobs, Transport and Resources, Bundoora, Victoria, Australia.
PLoS One. 2017 Mar 21;12(3):e0174176. doi: 10.1371/journal.pone.0174176. eCollection 2017.
This study investigated the usefulness of floral characters as a potential indicator of breeding system in the Brassicaceae. Initially, pod set, seed set and pollen tube growth experiments were carried out to confirm the breeding systems of 53 lines representing 25 different cultivated and weedy species from the Brassicaceae. The results of the pod set tests clearly differentiated between self-compatible and self-incompatible species. Floral characters were then evaluated on one or more lines of each of the 25 species. Fourteen floral characters were evaluated including, flower diameter, Cruden's outcrossing index, timing and direction of dehiscence and pollen-ovule ratio. Significant differences between species were evident in all of the floral characteristics evaluated. Flower diameter was generally larger in self-incompatible species than self-compatible species and pollen/ovule ratio was generally higher in self-incompatible species than self-compatible species. However, none of the floral characteristics was able to clearly differentiate the self-compatible and self-incompatible species and allow prediction of the breeding system with absolute confidence. The floral characteristic which was most effective at differentiating the two groups was anther direction at dehiscence.
本研究调查了花部特征作为十字花科植物潜在育种系统指标的实用性。最初，进行了结荚、结实和花粉管生长实验，以确认代表十字花科25个不同栽培种和杂草种的53个品系的育种系统。结荚试验结果清楚地区分了自交亲和与自交不亲和的物种。然后对25个物种中的每个物种的一个或多个品系的花部特征进行了评估。评估了14个花部特征，包括花直径、克鲁登杂交指数、开裂时间和方向以及花粉-胚珠比。在所评估的所有花部特征中，物种间存在显著差异。自交不亲和物种的花直径通常比自交亲和物种大，自交不亲和物种的花粉/胚珠比通常比自交亲和物种高。然而，没有一个花部特征能够清楚地区分自交亲和与自交不亲和的物种并绝对有把握地预测育种系统。在区分这两组时最有效的花部特征是花药开裂方向。
