Kendrick K E, Reznikoff W S
Department of Biochemistry, University of Wisconsin, Madison 53706.
J Bacteriol. 1988 Apr;170(4):1965-8. doi: 10.1128/jb.170.4.1965-1968.1988.
A transposition system constructed to detect the transposition of Tn5 to a site upstream of the lacZ gene has revealed that transposition of IS50L can activate downstream genes. Expression is apparently mediated by the NPTII promoter. Transposase produced either by IS50R or by the suppressed IS50L catalyzed transposition of IS50L.
构建用于检测Tn5转座至lacZ基因上游位点的转座系统已表明,IS50L的转座可激活下游基因。表达显然由NPTII启动子介导。由IS50R或受抑制的IS50L产生的转座酶催化IS50L的转座。