克隆的噬菌体T4 dCMP羟甲基化酶基因的表达及DNA序列
Expression and DNA sequence of the cloned bacteriophage T4 dCMP hydroxymethylase gene.
作者信息
Thylén C
机构信息
Department of Microbiology, Faculty of Pharmacy, Uppsala University, Sweden.
出版信息
J Bacteriol. 1988 Apr;170(4):1994-8. doi: 10.1128/jb.170.4.1994-1998.1988.
Abstract
A plasmid expressing the cloned bacteriophage T4 gene 42 gave the same levels of complementation of gene 42 mutants in a polarity-suppressing rho mutant as in a rho+ host. A reading frame likely corresponding to gene 42 and putative promoter and terminator sequences were identified in the partial sequence of the cloned fragment.
摘要
一个表达克隆的噬菌体T4基因42的质粒，在极性抑制rho突变体中对基因42突变体的互补水平与在rho+宿主中相同。在克隆片段的部分序列中鉴定出一个可能对应于基因42的阅读框以及推定的启动子和终止子序列。
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