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克隆的噬菌体T4 dCMP羟甲基化酶基因的表达及DNA序列

Expression and DNA sequence of the cloned bacteriophage T4 dCMP hydroxymethylase gene.

作者信息

Thylén C

机构信息

Department of Microbiology, Faculty of Pharmacy, Uppsala University, Sweden.

出版信息

J Bacteriol. 1988 Apr;170(4):1994-8. doi: 10.1128/jb.170.4.1994-1998.1988.

DOI:10.1128/jb.170.4.1994-1998.1988
PMID:2832395
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC211069/
Abstract

A plasmid expressing the cloned bacteriophage T4 gene 42 gave the same levels of complementation of gene 42 mutants in a polarity-suppressing rho mutant as in a rho+ host. A reading frame likely corresponding to gene 42 and putative promoter and terminator sequences were identified in the partial sequence of the cloned fragment.

摘要

一个表达克隆的噬菌体T4基因42的质粒，在极性抑制rho突变体中对基因42突变体的互补水平与在rho+宿主中相同。在克隆片段的部分序列中鉴定出一个可能对应于基因42的阅读框以及推定的启动子和终止子序列。

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EMBO J. 1999 Mar 1;18(5):1104-13. doi: 10.1093/emboj/18.5.1104.

本文引用的文献

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Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.缓冲液梯度凝胶和35S标记辅助快速DNA序列测定。
Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963-5. doi: 10.1073/pnas.80.13.3963.
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Escherichia coli promoter sequences predict in vitro RNA polymerase selectivity.大肠杆菌启动子序列可预测体外RNA聚合酶的选择性。
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The generation and analysis of clones containing bacteriophage T4 DNA fragments.包含噬菌体T4 DNA片段的克隆的产生与分析。
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Tetrahydrofolate-dependent labilization of the hydrogen atom on carbon 5 of 5'-deoxycytidylate, a step in the deoxycytidylate hydroxymethylase reaction.四氢叶酸依赖的5'-脱氧胞苷酸5位碳原子上氢原子的不稳定化，这是脱氧胞苷酸羟甲基化酶反应中的一个步骤。
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9
Host specificity of DNA produced by Escherichia coli: bacterial mutations affecting the restriction and modification of DNA.大肠杆菌产生的DNA的宿主特异性：影响DNA限制与修饰的细菌突变
J Mol Biol. 1966 Mar;16(1):118-33. doi: 10.1016/s0022-2836(66)80267-x.
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Pre-replicative development of the bacteriophage T4: RNA and protein synthesis in vivo and in vitro.
J Mol Biol. 1971 Sep 14;60(2):365-88. doi: 10.1016/0022-2836(71)90300-7.