Cai Yanling, Strømme Maria, Welch Ken
Division for Nanotechnology and Functional Materials, Department of Engineering Sciences, The Ångström Laboratory, Uppsala University, Box 534, 75121, Uppsala, Sweden.
3 Biotech. 2014 Apr;4(2):149-157. doi: 10.1007/s13205-013-0137-1. Epub 2013 May 21.
The aim of the present work was to evaluate several methods for analyzing the viability of bacteria after antibacterial photocatalytic treatment. Colony-forming unit (CFU) counting, metabolic activity assays based on resazurin and phenol red and the Live/Dead BacLight™ bacterial viability assay (Live/Dead staining) were employed to assess photocatalytically treated Staphylococcus epidermidis and Streptococcus mutans. The results showed conformity between CFU counting and the metabolic activity assays, while Live/Dead staining showed a significantly higher viability post-treatment. This indicates that the Live/Dead staining test may not be suitable for assessing bacterial viability after photocatalytic treatment and that, in general, care should be taken when selecting a method for determining the viability of bacteria subjected to photocatalysis. The present findings are expected to become valuable for the development and evaluation of photocatalytically based disinfection applications.
本研究的目的是评估几种分析抗菌光催化处理后细菌活力的方法。采用菌落形成单位（CFU）计数、基于刃天青和酚红的代谢活性测定以及Live/Dead BacLight™细菌活力测定（活/死染色）来评估经光催化处理的表皮葡萄球菌和变形链球菌。结果表明CFU计数与代谢活性测定结果相符，而活/死染色显示处理后细菌活力显著更高。这表明活/死染色试验可能不适用于评估光催化处理后的细菌活力，并且一般来说，在选择测定光催化处理后细菌活力的方法时应谨慎。本研究结果有望对基于光催化的消毒应用的开发和评估具有重要价值。
