Gahlout Mayur, Gupte Shilpa, Gupte Akshaya
Natubhai V. Patel College of Pure and Applied Sciences, Vallabh Vidyanagar, 388 120, Gujarat, India.
Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied Sciences, New Vallabh Vidyanagar, 388 121, Gujarat, India.
3 Biotech. 2013 Apr;3(2):143-152. doi: 10.1007/s13205-012-0079-z. Epub 2012 Aug 7.
The strain Ganoderma cupreum AG-1 (Genbank accession no. HQ328947) isolated from the decayed wood was evaluated for its ability to decolorize azo dye reactive violet 1 as well as for the production of ligninolytic enzymes. In the initial decolorization study, the strain was capable of decolorizing 19 different azo dyes. The strain was capable of decolorizing dye over a pH range of 4.5-6 at 30 °C. The optimum pH was found to be 4.5. Various other process parameters like additional carbon and nitrogen source and initial dye concentration were also optimized. The decolorization medium was supplemented with appropriate nitrogen source (yeast extract, 5 g l) and carbon source (mannose, 2 g l); the decolorization obtained was 98 %. The pattern of enzymes involved in the biodegradation was studied and laccase and MnP were found to be the major enzymes. High laccase activity shown by G. cupreum AG-1 and its ability to decolorize dyes are a good indication of its possible use in the treatment of textile effluents.
从腐朽木材中分离出的菌株铜绿灵芝AG-1（Genbank登录号HQ328947），对其对偶氮染料活性艳紫1的脱色能力以及木质素降解酶的产生情况进行了评估。在最初的脱色研究中，该菌株能够使19种不同的偶氮染料脱色。该菌株在30℃、pH值为4.5 - 6的范围内能够使染料脱色。发现最佳pH值为4.5。还对其他各种工艺参数，如额外的碳源和氮源以及初始染料浓度进行了优化。在脱色培养基中添加了合适的氮源（酵母提取物，5 g·l）和碳源（甘露糖，2 g·l）；获得的脱色率为98%。研究了参与生物降解的酶的模式，发现漆酶和锰过氧化物酶是主要的酶。铜绿灵芝AG-1显示出的高漆酶活性及其使染料脱色的能力，很好地表明了其在处理纺织废水方面的潜在用途。
