重组PhoE孔蛋白的高分辨率电子衍射

High-resolution electron diffraction of reconstituted PhoE porin.

作者信息

Jap B K

机构信息

Donner Laboratory, Lawrence Berkeley Laboratory, University of California, Berkeley 94720.

出版信息

J Mol Biol. 1988 Jan 5;199(1):229-31. doi: 10.1016/0022-2836(88)90393-2.

DOI:10.1016/0022-2836(88)90393-2

PMID:2832608

Abstract

PhoE porin has been reconstituted with phospholipid, forming large membrane patches. Electron diffraction shows that the reconstituted PhoE porin forms highly coherent crystalline arrays, giving structural information to a resolution of 3.4 A. The crystal form is of the orthorhombic space group P2(1)2(1)2, with unit cell dimensions a = 150 A and b = 129 A. Images of negatively stained PhoE crystalline patches show that there are four PhoE porin trimers in a unit cell.

摘要

PhoE孔蛋白已与磷脂重组，形成大的膜片。电子衍射表明，重组后的PhoE孔蛋白形成了高度相干的晶体阵列，提供了分辨率为3.4埃的结构信息。晶体形式为正交空间群P2(1)2(1)2，晶胞尺寸a = 150埃，b = 129埃。负染的PhoE晶体膜片图像显示，一个晶胞中有四个PhoE孔蛋白三聚体。