表达人类受体、细胞周期蛋白T1和CRM1基因的转基因大鼠源原代巨噬细胞/T细胞及T细胞系对HIV-1的易感性。

HIV-1 susceptibility of transgenic rat-derived primary macrophage/T cells and a T cell line that express human receptors, CyclinT1 and CRM1 genes.

作者信息

Shida Hisatoshi, Okada Hiroyuki, Suzuki Hajime, Zhang Xianfeng, Chen Jing, Tsunetsugu-Yokota Yasuko, Tanaka Yuetsu, Yakushiji Fumika, Hayashi Yoshio

机构信息

Institute for Genetic Medicine, Hokkaido University, Kita-ku, Sapporo, 060-0815, Japan.

Department of Medical Technology, Tokyo University of Technology, 5-23-22 Nishikamata, Ohta-ku, Tokyo, 144-8535, Japan.

出版信息

Genes Cells. 2017 May;22(5):424-435. doi: 10.1111/gtc.12486. Epub 2017 Mar 22.

DOI:10.1111/gtc.12486

PMID:28326644

Abstract

We developed transgenic (Tg) rats that express human CD4, CCR5, CXCR4, CyclinT1, and CRM1 genes. Tg rat macrophages were efficiently infected with HIV-1 and supported production of infectious progeny virus. By contrast, both rat primary CD4 T cells and established T cell lines expressing human CD4, CCR5, CyclinT1, and CRM1 genes were infected inefficiently, but this was ameliorated by inhibition of cyclophilin A. The infectivity of rat T cell-derived virus was lower than that of human T cell-derived virus.

摘要

我们培育出了表达人类CD4、CCR5、CXCR4、细胞周期蛋白T1和CRM1基因的转基因（Tg）大鼠。Tg大鼠巨噬细胞能被HIV-1有效感染，并支持产生有传染性的子代病毒。相比之下，大鼠原代CD4 T细胞以及表达人类CD4、CCR5、细胞周期蛋白T1和CRM1基因的已建立T细胞系被感染的效率较低，但通过抑制亲环素A可改善这种情况。大鼠T细胞衍生病毒的感染性低于人类T细胞衍生病毒。