Zheshan Zhao, Hua Huang, Rongmin Qiu, Kezhen Wei, Limei Li
Dept. of Pediatric Dentistry, Hospital of Stomatology, Guangxi Medical University, Nanning 530021, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2016 Oct 1;34(5):463-467. doi: 10.7518/hxkq.2016.05.006.
To study the biocompatibility of the polylactic acid (PLA)-absorbable root post film prepared by solvent evaporation film.
The PLA post film-leaching liquor was prepared by the solvent evaporation of PLA root post films, and its biocompatibility was measured. Sources of human gingival fibroblasts (HGF) were cultivated in vitro and identified initially by the immunohistochemistry method. The toxicity reaction, survival rate, and morphological change of HGF incubated in PLA post film-leaching liquor were observed and tested by morphological observation, 3-(4,5-dimethylthiazolyl)-2,5-diphenyltetrazolium bromide (MTT) assay, and flow cytometry. The HGF were implanted on the PLA post film and observed by 4',6-diamidino-2-phenylindole staining. After implanting the PLA post film, the surrounding subcutaneous tissue of SD rats and the implants were removed from the back of the rats in the 1st, 4th, 8th, and 12th weeks to observe the general condition of the incisions. The organization was given the hematoxylin-eosin (HE) stain to find the organization condition of local tissue inflammation and foreign body reaction through the infiltration degree of inflammatory fibroblasts.
The effects of the PLA post film-leaching liquor on the proliferation of HGF and fibroblast toxicity were insignificant. The living fibroblast rate was similar to the normal control group. The HGF were able to grow on the PLA film. The results of the organization general observation and HE staining of the rats were similar to the results for the control group, which met all the demands made by biological safety.
The PLA-absorbable root post film prepared by the solvent evaporation film has good biocompatibility and can be used for further clinical research.
研究溶剂挥发法制备的聚乳酸（PLA）可吸收牙根桩膜的生物相容性。
通过溶剂挥发法制备PLA桩膜浸提液，并测定其生物相容性。体外培养人牙龈成纤维细胞（HGF），采用免疫组织化学方法进行初步鉴定。通过形态学观察、噻唑蓝（MTT）比色法及流式细胞术观察并检测PLA桩膜浸提液中HGF的毒性反应、存活率及形态变化。将HGF接种于PLA桩膜上，采用4',6-二脒基-2-苯基吲哚染色观察。在植入PLA桩膜后第1、4、8和12周，从SD大鼠背部取出周围皮下组织及植入物，观察切口的一般情况。对组织进行苏木精-伊红（HE）染色，通过炎性成纤维细胞的浸润程度了解局部组织炎症及异物反应的组织学情况。
PLA桩膜浸提液对HGF增殖及成纤维细胞毒性作用不明显。存活的成纤维细胞率与正常对照组相似。HGF能够在PLA膜上生长。大鼠组织大体观察及HE染色结果与对照组相似，符合生物安全性的各项要求。
溶剂挥发法制备的PLA可吸收牙根桩膜具有良好的生物相容性，可用于进一步的临床研究。
