Department of Surgery, Cardiovascular Institute & Fuwai Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
ASAIO J. 2012 May-Jun;58(3):262-7. doi: 10.1097/MAT.0b013e31824709ee.
A novel poly(butyl succinate) (PBS) and polylactic acid (PLA) blend with the favorable mechanical and degradable properties may be applied in the field of biomedicine. The purpose of this study was to investigate the in vitro and in vivo biocompatibilities of the PBS/PLA blend for future application. For in vitro analysis, the L929 fibroblasts and bone marrow stem cells (BMSCs) had been chosen to assess the cytocompatibility. The extract of PBS/PLA blend did not show any cytotoxicity to the L929 and BMSCs by Cell Counting Kit-8 and lactate dehydrogenase assays. Meanwhile, the results of the cytocompatibility showed no difference between the PBA/PLA blend and pure PLA and PBS. Subsequently, they were implanted into rats subcutaneously for in vivo study. It was found that similar with pure PLA and PBS, PBS/PLA blend caused the mild inflammation and foreign body reaction in rats during the consecutive 9 month implantation. However, the status of fibrosis surrounding PBS/PLA blend was superior to the pure PLA and PBS. In all, it was demonstrated that the novel PBS/PLA blend had excellent biocompatibilities in vitro and in vivo.
一种具有良好机械性能和可降解性能的新型聚丁二酸丁二醇酯（PBS）和聚乳酸（PLA）共混物可能应用于生物医学领域。本研究旨在研究 PBS/PLA 共混物的体外和体内生物相容性，以用于未来的应用。体外分析中，选用 L929 成纤维细胞和骨髓基质干细胞（BMSCs）评估细胞相容性。通过细胞计数试剂盒-8 和乳酸脱氢酶测定，PBS/PLA 共混物的浸提液对 L929 和 BMSCs 均无细胞毒性。同时，细胞相容性结果表明，PBS/PLA 共混物与纯 PLA 和 PBS 之间没有差异。随后，将其植入大鼠皮下进行体内研究。结果发现，与纯 PLA 和 PBS 相似，PBS/PLA 共混物在连续 9 个月的植入过程中引起大鼠轻度炎症和异物反应。然而，PBS/PLA 共混物周围纤维组织的状态优于纯 PLA 和 PBS。总之，该新型 PBS/PLA 共混物具有良好的体外和体内生物相容性。
