He Pei, Yang Peisong, Tang Shiyun, Ye Ling, Liu Chunbo, Shen Qinpeng, Tang Dangyu, Zhang Fengmei, Liu Zhihua, Chen Yongkuan, Yao Shouzhuo, Xiang Nengjun, Huang Zhangjie
a Key Laboratory of Tobacco Chemistry of Yunnan Province , China tobacco yunnan industrial Co., Ltd , Kunming , P.R. China.
b School of Chemical Science and Technology , Yunnan University , Kunming , P.R. China.
Nat Prod Res. 2017 Dec;31(23):2730-2736. doi: 10.1080/14786419.2017.1292512. Epub 2017 Mar 22.
Three new isobenzofurans (1-3), together with four known phenylpropanoids (4-7) were isolated from the roots of Nicotiana tabacum. Their structures were determined by means of HRESIMS and extensive 1D and 2D NMR spectroscopic studies. Compounds 1-6 were tested for their anti-tobacco mosaic virus (anti-TMV) activities and cytotoxicity activities. The results showed that compounds 5 and 6 exhibited high anti-TMV activities with inhibition rates of 35.1 and 33.4%, respectively. The cytotoxicities of compounds 1-7 against five human tumor cell lines (NB4, A549, SHSY5Y, PC3 were also tested. Compounds 1-7 showed weak inhibitory activities against some tested human tumor cell lines with IC values in the range of 3.8-9.6 μM.
从烟草的根部分离出三种新的异苯并呋喃（1-3）以及四种已知的苯丙素类化合物（4-7）。通过高分辨电喷雾电离质谱（HRESIMS）以及广泛的一维和二维核磁共振光谱研究确定了它们的结构。测试了化合物1-6的抗烟草花叶病毒（抗TMV）活性和细胞毒性活性。结果表明，化合物5和6表现出较高的抗TMV活性，抑制率分别为35.1%和33.4%。还测试了化合物1-7对五种人类肿瘤细胞系（NB4、A549、SHSY5Y、PC3）的细胞毒性。化合物1-7对一些测试的人类肿瘤细胞系显示出较弱的抑制活性，IC值在3.8-9.6μM范围内。
